芳维A酸乙酯对Hut-78细胞增殖及凋亡的影响

Effects of Arotinoid Ethylester on Proliferation and Apoptosis of Hut-78 Cells

目的观察并评价芳维A酸乙酯对皮肤T细胞淋巴瘤细胞(Hut-78)增殖及凋亡的影响。方法用不同浓度的维甲酸分别处理Hut-78细胞,用四甲基偶氮唑盐比色法(methyl thiazolyl tetrazolium,MTT)检测细胞增殖的抑制率、流式细胞仪检测细胞凋亡情况。结果经浓度为10-8 mol/L、10-7 mol/L、10-6 mol/L、10-5 mol/L的维甲酸分别作用于Hut-78细胞48 h,对细胞增殖的抑制率分别为(10.83±1.55)%、(17.71±3.60)%、(25.89±1.08)%、(30.92±1.86)%。用10-7 mol/L的维甲酸分别作用于Hut-78细胞12、24、36、48 h,其抑制率分别为(4.87±1.87)%、(10.87±0.77)%、(15.61±1.66)%、(19.57±1.80)%、(27.98±1.86)%。经浓度为10-7、10-5 mol/L的维甲酸作用48 h,Hut-78细胞的凋亡率分别为(11.56±1.51)%、(20.48±2.34)%。结论芳维A酸乙酯能够明显抑制Hut-78的增殖并能诱导细胞凋亡。

Objective To observe and evaluate the effects of arotinoid ethylester on proliferation and apoptosis of T lymphoma cells（Hut-78 cells）.Methods Hut-78 cells were treated with different concentrations of arotinoid ethylester.Hut-78 cell proliferation and apoptosis was assessed by methyl thiazolyl tetrazolium（MTT） assay and Annexin V/PI doubled-labeled flow cytometry respectively.Results The inhibitory rate of Hut-78 cells was（10.83±1.55）%,（17.71±3.60）%,（25.89±1.08）%,（30.92±1.86）% respectively after the treatment with arotinoid ethylester at the concentration of 10-8,10-7,10-6,10-5 mol/L for 48 hours.The inhibitory rate of Hut-78 cells was（4.87±1.87）%,（10.87±0.77）%,（15.61±1.66）%,（19.57±1.80）%,（27.98±1.86）% respectively after the treatment with 10-7 mol/L arotinoid ethylester for 12,24,36 and 48 hours.The apoptotic rate of Hut-78 cells was（11.56±1.51）% and（20.48±2.34）% respectively after the treatment with arotinoid ethylester at the concentration of 10-7 and 10-5mol/L for 48 hour.Conclusion Arotinoid ethylester can significantly inhibit the proliferation and induce the apoptosis of Hut-78 cells.