3-[二(羧甲基)氨甲基]-1,2-二羟基蒽醌与蛋白质作用的研究

The Study on the Reaction between 3 - [Di(carboxymethyl)aminomethyl] -1,2- Dihydroxyanthraquinone and Bovine Serum Albumin

在pH4.1的缓冲溶液中,研究3-[二(羧甲基)氨甲基]-1,2-二羟基蒽醌(AFB)与牛血清蛋白(BSA)结合反应,相互生成紫色的配合物.采用UV光谱法,测得此配合物的最大吸收峰值为510nm,与试剂本身相比较红移90nm.表观摩尔吸光系数ε=1.2474×10~4mol^(-1)·cm^(-1)·L,最大结合数n=7,表观结合常数K_C=3.85×l0~6.研究发现,AFB与BSA之间主要是以分子之间的静电引力结合,认为该反应符合Scatchard模型.离子强度对结合反应有显著的影响.

The interaction of 3 - [di(carboxymethyl)aminomethyl] - 1,2 - dihydroxyanthraquinone (AFB) and bovine serum albumin (BSA) was investigated by spectrophotometric method in acidic solution (pH4.1) . When BSA was added into AFB solution, a purple color was observed, which indicated formation the of the AFB - BSA complex. The maximum absorption of the complex was obtained at 510nm, with the apparent molarabsorptivity of e = 1. 2474 × 104mol-1·cm-1·L. Two conditional constants were defined: the maximumbinding number n = 7 and apparent binding constant KC = 3.85 × 106. It is suggested that the electrostatic force is the main binding force. The Scatchard model is appropriate in the treatment of data obtained here. It is found that sodium chloride concentration of the solution has significant effect on the sensitivity of AFB - BSA complex.