氧代赖氨酸对持瘤小鼠免疫功能影响的初步研究

PRIMARY STUDIES ON OXALYSINE ON THE IMMUNE RESPONSE OF TUMOR-BEARING MICE

用Winn试验观察氧代赖氨酸对持瘤小鼠免疫功能的影响,结果显示经药物治疗后持瘤小鼠的脾细胞与瘤细胞温育后再接种之平均瘤重明显低于未经处理的对照组,p<0.05,提示氧代赖氨酸能部分激活持瘤小鼠脾脏中的某些免疫活性细胞。用α-萘酚醋酸酯酶染色法观察小鼠外周血及脾脏中T淋巴细胞的含量。小鼠接种肿瘤后第3、6、9天ANAE^+ T淋巴细胞值与正常小鼠相比无明显变化(p>0.05)。经氧代赖氨酸(100、150、200mg/kg)治疗,所得结果相似,也无明显变化。

Oxalysine is an antitumor antibiotic and showed antitumor activity against various animal solid tumors. In the present paper, the influence of oxalysine on the immune respones of tumor-bearing mice was studied.A neutralization activity against tumor cell was observed by Winn method using spleen cell of tumor-bearing mice which had been pretreated with oxalysine 200 mg/ kg ip× 7 .The mixture of spleen and tumor cell was s.c.into the mice and a mean tumor weight of 0.5±0.3g was observed. In untreated control the mean tumor weight was 1.0±0.5g. Hence oxalysine possesses a slight immune stimulation on the spleen cell of tumor-bearing mice.T lymphocyte count was determined by histochemical staining technique using acid α-naphthyl acetate esterase. The mean count of esterase-positive lymphocytes obtained from spleen or blood were determined. On day 3, day 6 , day 9 after implantation of tumor the number of esterase-positive lymphocytes was determined and no significant change was noted.The same result was also obtained when oxalysine was ad-ministred with various doses ranging from 100 to 200 mg/kg.