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铁调素对体外多巴胺能神经细胞内铁聚积的作用 被引量：3

The Role of Hepcidin in Dopamine Nerve Cell in Vitro
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摘要 目的:探讨铁调素在多巴胺能神经细胞内铁代谢调节的作用。方法:体外培养MES23.5细胞,经用枸橼酸铁胺处理4 h后应用RT-PCR检测铁调素mRNA的表达,Western blot检测膜铁转运蛋白1(FPN1)的表达。结果:高铁作用4 h后观察到MES23.5细胞内铁调素表达增高,FPN1表达降低(P<0.05)。结论:铁调素通过减少FPN1影响多巴胺能神经元的铁转出,参与帕金森病的铁聚积。 Aim： To study the activation of hepcidin（a iron-regulatory hormone） on intracellular iron regulatory.Methods： After the treatment with 100 μmol/L ferric ammonium citrate（FAC） for 4 h in vitro,hepcidin mRNA levels were detected by reverse transcription-polymerase chain reaction and FPN1 protein levels were determined by Western blot.Results： In dopaminergic cell line MES23.5 cells,the expression levels of hepcidin were observed up-regulated（P0.05） and FPN1 protein levels lower（P0.05）.Conclusion： Hepcidin restrict iron release by down-regulating FPN1 expression,contributing to the abnormal iron accumulation of Parkinson＇s disease.
作者 孙超 宋宁 朱德璋 谢安木
机构地区 青岛大学医学院附属医院东区神经内科 青岛大学医学院生理教研室 青岛大学医学院附属医院麻醉科
出处 《中国临床神经科学》 2012年第2期176-180,共5页 Chinese Journal of Clinical Neurosciences
关键词 铁调素 膜铁转运蛋白1 MES23.5细胞 铁聚积 hepcidin ferroportin 1 MES 23.5 cell iron accumulation
分类号 R742.5 [医药卫生—神经病学与精神病学]
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参考文献23

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共引文献28

同被引文献37

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引证文献3

二级引证文献18

中国临床神经科学
2012年 第2期

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