牛流行热病毒JB76H株G蛋白基因核苷酸序列分析

Nucleotide Sequence of G Protein Gene of Bovine Ephemeral Fever Rhabdovirus JB76H Strain

通过反转录_聚合酶链反应(RT_PCR),从中国北京分离株牛流行热病毒JB76H 基因组RNA中扩增出主要保护性抗原糖蛋白G的cDNA。采用Sanger’s 双脱氧末端终止法测定cDNA 片段的核苷酸序列,并推导出氨基酸序列。G基因全长为1872 个碱基,单一的开放阅读框架编码623 个氨基酸的多肽。将测得的序列与澳大利亚六个分离株进行比较,发现我国分离株与澳大利亚分离株间同源性为91% ,低于澳大利亚各分离株之间的同源性(98% ～99% ) 。同时氨基酸序列比较结果也显示,我国分离株与澳大利亚分离株间同源性(93.9% )低于澳大利亚各分离株之间的同源性(96% ～98 %) 。

The surface glycoprotein G gene of Bovine ephemeral fever virus(BEFV)JB76H was amplified by reverse transcriptase polymerase chain reaction(RT_PCR)and cloned into Bluescript M13 _,The sequence of the cDNA was obtained by Sanger's sequencing technique with their amino acid sequence being deduced. The results indicated that G gene is 1872bp in length and has a single open reading frame which codes a polypeptide of 623 amino acid. G glycoprotein has two highly hydrophobic domains. Comparison of nucleotide and amino acid sequences showed that the homologies were about 91% and 93.9% between Chinese and Australia isolates. Comparatively, this means that Chinese isolates were far distant from those Australia isolates. The surface glycoprotein G gene of Bovine ephemeral fever virus(BEFV)JB76H was amplified by reverse transcriptase polymerase chain reaction(RT_PCR)and cloned into Bluescript M13 _,The sequence of the cDNA was obtained by Sanger's sequencing technique with their amino acid sequence being deduced. The results indicated that G gene is 1872bp in length and has a single open reading frame which codes a polypeptide of 623 amino acid. G glycoprotein has two highly hydrophobic domains. Comparison of nucleotide and amino acid sequences showed that the homologies were about 91% and 93.9% between Chinese and Australia isolates. Comparatively, this means that Chinese isolates were far distant from those Australia isolates.