多脂鳞伞紫外线诱变育种

Ultraviolet mutation screening of Pholiota adipose

该研究用已经选育出来的多脂鳞伞菌株作为出发菌株,利用紫外线对其孢子悬液进行诱变处理。结果表明,在紫外线照射65秒的情况下,得到一株正突变菌株(UV-65),经过斜面培养和发酵实验证实,其菌丝生长比出发菌株旺盛、菌丝产量提高20%;经过6代的连续传代培养,其遗传性状较为稳定。

Using an obtained Pholiota adipose strain as starting strain,spore suspension was induced by ultraviolet（UV）.The results showed that a mutant strain UV-65 was obtained after 65s treatment under UV.It was demonstrated that UV-65 grew stronger than the starting strain and the production of mycelia increased by 20%.It had stable inheritance after six continuously passages.