苹果矮化中间砧SH40激素含量及生长素转运蛋白基因pin1表达

The content of hormone and auxin transport gene pin1 of SH40 as the interstock of apple

为探讨SH40作为苹果中间砧时激素含量及生长素转运蛋白基因pin1表达量变化与矮化的可能存在的关系,为今后致矮机理研究打下基础。在控制条件下,以矮化中间砧砧穗组合富士/SH40/八棱海棠、乔化自根砧砧穗组合富士/八棱海棠以及乔化苗八棱海棠和矮化苗SH40为试材,采用HPLC法测定分析了材料茎皮中生长素(IAA)和脱落酸(ABA)含量的变化,采用qRT-PCR研究了pin1基因表达量的变化。结果表明:无论砧木苗本身,还是嫁接品种后,SH40茎皮中ABA/IAA比值显著高于乔化砧木八棱海棠;SH40做中间砧时,其茎皮中IAA含量显著减少,可能与其中IAA转运蛋白pin1基因表达量显著降低有关。

Both rootstock SH40,Malus micromalus and scion-rootstock combinations Fuji/SH40/Malus micromalus,Fuji/Malus micromalus were used as the materials in this experiment,Both IAA and ABA were measured by HPLC in the bark of different materials.qRT-PCR（quantitative realtime PCR） were also used to search for the difference of expression of pin1 gene.Our aim was to explore the relationship between the content of hormone,the expression of pin1 gene and the possible dwarfing factors of SH40 as the interstock of apple.The results showed that the ratio of ABA/IAA was remarkable higher in the dwarf trees than the vigorous controls.In graft combination Fuji/SH40/Malus micromalus,the content of IAA in the bark of the interstock was much lower than the scion,which might be due to the significant lower expression of IAA-transporting protein gene pin1 in the bark of the interstock.