汉黄芩素固体脂质纳米粒的制备及体外释放度考察

Preparation and in vitro Drug Release of Wogonin-loaded Solid Lipid Nanoparticles

目的:制备汉黄芩素固体脂质纳米粒并对其体外释放度进行考察。方法:采用乳化分散-超声法制备汉黄芩素固体脂质纳米粒,以包封率和载药量为评价指标,进行正交试验筛选最优处方,并对最优处方的外观、粒径和体外释放度进行考察。结果:制得的纳米粒为均一球形,平均粒径为(153±34)mm,其平均载药量为(60.53±2.17)%,平均包封率为(85.54±4.16)%,48 h累积释放达80%。结论:本试验获得了较理想的汉黄芩素固体脂质纳米粒,其体外释放具有缓释作用。

Objective：To prepare wogonin-loaded solid lipid nanoparticles（SLN）and evaluate the drug release behavior in vitro. Method：The wogonin SLN were prepared by emulsification dispersion-ultrasonic method.Based on the results of single-factor screening experiment,the preparation methods were screened by orthogonal experiments.The mean diameter,size distribution and drug release in vitro were investigated.Result：The optimized SLN were spherical and uniform under a transmission electron microscopy.The average particle size was（153±34）nm,drug loading was（60.53±2.17）%and entrapment rate was（85.54±4.16）%.The drug release rate could reach 80%in 48 hours.Conclusion：The optimized preparation of wogonin SLN with sustained-release property is obtained successfully.