氟碳化合物与环糊精包合物的制备及其对牛血清白蛋白的影响

Preparation of Perfluorocarbon and Cyclodextrin Inclusion Complex and Its Effect on Bovine Serum Albumins

以氟碳化合物FC-77作为模型药物,制备FC-77与β-环糊精(β-CD)包合物。考察包合物与牛血清白蛋白(BSA)的相互作用。通过紫外-可见光谱、红外光谱、热重分析等方法研究包合物的物理化学性能。通过荧光光谱法研究包合物对BSA的荧光猝灭作用。结果为FC-77与β-CD之间存在强烈的相互作用,从而增加了FC-77的稳定性和水中的溶解性。荧光猝灭结果显示FC-77分子能进入BSA的疏水区域。结论是以β-CD为介质,能显著改善FC-77的理化性能,使FC-77粉末化,便于制成多种剂型,扩大给药方式。

FC - 77, a kind of perfluorocarbons, was used as model drug to prepare FC - 77/β - CD inclusion complex and study its effect on bovine serum albumins （BSA）. The physieochemical properties of FC -77/β- CD inclusion complex were characterized by UV - vis absorption spectra, FT - IR spectra and the thermogravimetrie thermal analysis. The interaction of FC - 77/β - CD inclusion complex and BSA was determined by fluorescence quenching technology in fluorescence methodology. FC -77 can be formed an optimal inclusion complex with eyelo- dextrin due to their strong interaction, which can improve the stability of FC -77 and increase their solubility in water. Furthermore, fluorescence quenching result shows that FC -77 can bind with BSA through FC -77 molecu-lar transferring to hydrophobic region of BSA.β - cyclodextrin, used as medium, can significantly change physico- chemical properties of FC - 77 and prepare powdery FC - 77, which result to the enlargement of the dosage and ad- ministration methods of FC -77.