微生物转位在HIV感染疾病进程中的作用

Microbial translocation in the progression of HIV infection

目的了解微生物转位在人类免疫缺陷病毒(HIV)感染疾病进程中的意义。方法用酶联免疫吸附(ELISA)法检测健康人和HIV感染者血浆脂多糖(LPS)水平;用流式细胞仪对HIV-1感染者进行CD4+T淋巴细胞计数和CD8+T淋巴细胞活化标志CD38表达水平检测;用Cobas全自动PCR系统检测HIV-1感染者病毒载量。结果 HIV-1感染者血浆LPS浓度为(21.89±12.56)ng/L,与健康对照(13.99±9.03)ng/L比较,水平显著升高,P=0.019;HIV-1感染者血浆LPS浓度与CD4+T淋巴细胞数目、病毒载量均无相关性,但与CD8+T淋巴细胞活化标志CD38表达水平呈正相关(r=0.457,P=0.016)。结论微生物转位可能是直接导致HIV感染相关的免疫活化发生的一个重要机制。

Objective To explore microbial translocation in the progression of HIV-1 infection. Methods Plasma levels of tipopolysaccharide （LPS） in healthy controls and HIV-1 infected individuals were determined by ELISA. Flow cytometry was performed to obtain absolute CD4 cell counts and the expression of CD38 in CD8＋ T cells in HIV-1 positive individuals. HIV viral loads were detected by Cobas TaqMan 48 real-time PCR analyzer. Results Plasma levels of LPS in HIV-1 infected individuals were significantly higher than that in healthy control [ （21.89±12.56） ng/L vs （13.99± 9.03） ng/L, P= 0. 019]. Plasma levels of LPS were correlated with the percentage of CD8＋ CD38＋ T lymphocytes （r= 0. 457, P= 0. 016） but neither with the absolute CD4 counts nor with the HIV-1 viral loads. Conclusions Microbial translocation may play a role in HIV-1 infection-associated systemic immune activation.