摘要
目的:探讨靛玉红甲肟(IRO)对人喉癌Hep-2细胞增殖的影响及机制。方法:MTT法检测不同浓度、不同作用时间IRO对Hep-2细胞增殖活性的影响。流式细胞仪检测IRO对Hep-2细胞周期的影响。Western法检测IRO对Hep-2细胞CDK4和cyclinD1蛋白表达的影响。结果:MTT结果发现IRO对Hep-2细胞具有明显的增殖抑制作用,且表现为剂量依赖性(P<0.01)。流式检测发现,以10μmol/L的IRO处理Hep-2细胞后,细胞阻滞于G0/G1期,不同浓度组间差异存在显著性(P<0.01)。Western结果发现IRO对Hep-2细胞CDK4和cyc-linD蛋白的表达有抑制作用(P<0.01)。结论:IRO具有抑制人喉癌Hep-2细胞增殖的作用,其机制与阻滞细胞于G0/G1期有关。
Objective： To study the effect of Indirubin - 3＇ - monoxime（IRO） on human laryngeal carcinoma Hep- 2 cells prolifera- tion and to explore its mechanism. Methods： Methabenzthia zuron（MTI＇） was used to ob- serve the proliferation of Hep -2 cells. Flow cytometry was used to measure the cell cycle of Hep -2 cells. Western blot was used to measure the expression of CDK4 and eyclin D1 protein in the Hep -2 cells. Results： IRO inhibited growth of Hep -2 cells in a dose - dependent and time - dependent manner（ P 〈0. Ol ）. IRO can arrests Hep- 2 cell at GO/G1 phase（P 〈0. 05）. The ex- pression of CDK4 and cyclin DI protein of Hep- 2 cells were decreased, There weresignificant differences between different time groups（ P 〈 0.01 ）. Conclusions： IRO can inhibit the human laryngeal carcinoma Hep -2 cells proliferation, its mechanism is re- lated to arrests Hep -2 cell at G0/G1 phase and reduced CDK4 and cyclin D1 expres- sion.
