直肠癌组织中PgP蛋白和p16蛋白的表达及临床意义

Expression of the PgP and p16 Protein in Rectum Cancer and its Clinical Significance

目的探讨PgP和p16蛋白在直肠癌组织中的表达情况及临床意义。方法应用MaxVisionTM免疫组化的方法对71例直肠癌及癌旁组织进行PgP和p16蛋白检测。结果 PgP的总阳性率为84.51%(60/71)。PgP的阳性表达与直肠癌的淋巴结有无转移有关,与癌组织的分化程度、浸润深度与伴不伴有神经浸润无关。其中低分化腺癌(93.33%,28/30)高于高分化腺癌(82.61%,19/23)、中分化腺癌(72.22%,13/18,P>0.05)。淋巴结无转移组93.48%(43/46)明显高于淋巴结转移组68%(17/25,P<0.05)。伴有神经浸润组85.71%(24/28)与不伴有神经浸润组(83.72%,36/43,P>0.05)两者之间差异无统计学意义。癌组织浸润到浆膜外脂肪组织(87.5%,21/24)高于浸润到肠壁全层(84.21%,32/38),高于浸润到肠壁肌层(77.78%,7/9,P>0.05)。p16蛋白的总阳性率为(64.79%,46/71)。p16蛋白的阳性表达与淋巴结有无转移及组织的分化程度无关,与伴不伴有神经浸润及癌组织的浸润深度有关。其中其低分化腺癌(73.33%,22/30)高于高分化腺癌(60.87%,14/23)和中分化腺癌(55.56%,10/18,P>0.05)。淋巴结转移组(17/25,68%)与淋巴结无转移组(29/46,63.04%)两者之间差异无统计学意义(P>0.05)。不伴有神经浸润组(79.07%,34/43)明显高于伴有神经浸润组(42.86%,12/28,P<0.05)。癌组织浸润到肌层(88.89%8/9)高于浸润到全层(71.05%,27/38),明显高于癌组织浸润到浆膜外脂肪组织(45.83%,11/24,P<0.05)。结论 PgP和p16蛋白的阳性表达与直肠癌的某些临床病理指标相关,可作为对判断直肠癌的恶性程度,预测肿瘤侵袭转移,评估预后的良好指标。

Objective To determine the expression of PgP,pl6 protein and carcinogenesis and progression of rectum cancer. Methods PgP and p16 expression in 71 eases of rectum cancer and the surrounding cancer tissue was detected by MaxVisionTM immunohistochemical method. Results Position rate of PgP in rectum cancer was 84.51% （ 60/71 ）. PgP positive rate was corre lated with lymph nodes metastases and was not correlated with differentiation of progression, the depth of tumor and nervous im pregnation. The percentage of PgP in poorly differentiated carcinomas（93.33% ,28/30） was higher than that in well and moder ately differentiated tumors （82.61%, 19/23;72. 22%, 13/18）. PgP positive rate in tumors without lymph nodes metastases （93.48% ,43/46 ） was higher than that with metastases （68 % , 17/25 ）. There was no obvious difference between neural invasion and no nerve infiltration （85.71% ,24/28;83.72% ,36/43 ;P 〉 0.05 ）. Cancer invasion to serosa outside adipose tissue was high er than that into the intestinal wall layer and the muscular layer（ 87.5 % ,21/24;84.21% ,32/38 ;77.78% ,7/9 ;P 〉 0.05 ）. Po sition rate of p16 in rectum cancer was 64.79% （46/71）. p16 positive rate was not correlated with lymph nodes metastases and differentiation of progression, p16 positive rate was correlated with the depth of tumor and nervous impregnation. The percentage of p16 in poorly differentiated carcinomas was higher than that in well and moderately differentiated tumors （73.33% , 22/30; 60.87% , 14/23 ;55.56% , 10/18 ;P 〉 0.05 ）. There was no obvious difference between lymph nodes metastases and without me tastases（68% , 17/25 ;63.04% ,29/46 ;P 〉 0.05 ）. p16 position rate in no neural invasion was much higher than that invasion （79.07% ,34/43 ;42.86% , 12/28 ;P 〈 0.05 ）. Carcinoma infiltrating the muscular layer was higher than that into the intestinal wall layer and into serosa outside adipose tissue（88.87% ,8/9;71.05% ,27/38;45.83% ,11/24;P〈0.05）. Conclusion PgP and p16 protein position were correlated with some clinical pathological factors. The PgP and p16 protein can be used to evaluate the biological behavior and prognosis of rectum cancer.