肾细胞癌患者手术前后外周血T淋巴细胞亚群的变化及其临床意义

Variation and clinical significance of T lymphocyte subsets in peripheral blood of patients with renal cell carcinoma

目的通过检测外周血T淋巴细胞亚群的水平,观察肾细胞癌患者治疗前后免疫功能的变化规律,探讨其在诊断及疗效判断上的意义。方法选取2006～2009年肾细胞癌(renal cell carcinoma,RCC)患者39例,应用流式细胞技术检测术前及术后外周血CD3+、CD4+、CD8+、CD4+/CD8+,并与健康对照组进行对比研究。结果肾细胞癌患者术前CD3+、CD4+、CD4+/CD8+低于健康对照组,术后患者CD3+、CD4+、CD4+/CD8+较术前明显升高。结论肾细胞癌患者术前细胞免疫功能受到不同程度的抑制,检测外周血T淋巴细胞亚群,对评估患者的细胞免疫功能状况、疾病的诊断和疗效观察具有积极的临床意义。

Objective To observe the post-operative variation of immune function in patients with renal cell carcinoma and to investigate the significance of diagnosis and therapeutic effect evaluation by detecting the T-lymphocyte subset level in the peripheral blood of patients with renal cell carcinoma.Methods Thirty-nine patients with renal cell carcinoma were recruited between 2006 and 2009.The level of CD3＋,CD4＋,CD8＋,and CD4＋/CD8＋ in the peripheral blood of these patients was detected by flow cytometry before and after operation and compared with that in control group.Results The level of CD3＋、CD4＋ and CD4＋/CD8＋ in patients with renal cell carcinoma was lower than in control group while the post-operative level of CD3＋、CD4＋ and CD4＋/CD8＋ was significantly higher.Conclusions The pre-operative immune function in patients with renal cell carcinoma is inhibited to some extent.Detecting the T-lymphocyte subsets level in the peripheral blood of patients with renal cell carcinoma is of positive clinical significance for evaluation of the cell immue status,diagnosis of the disease and observation of the therapeutic effect.