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腹腔注射酵母多糖后小鼠脾脏Foxp3的变化 被引量：1

Changes of Foxp3 in Splenocytes of Mouse after the Abdominal Injection of Zymosan
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摘要 目的:通过观察小鼠症状和脾脏大小及脾脏中Foxp3的变化,阐述脾脏Foxp3在酵母多糖(zymosan)所致炎性反应中的作用。方法:采用免疫荧光染色检测正常和腹腔注射酵母多糖后各个时间点小鼠脾脏中Foxp3的变化过程。结果:小鼠6h出现中毒症状,1d,2d时症状最重,死亡率最高;Foxp3在脾脏中的表达在6h时开始减少,3d最少,5d时开始增加(P<0.05)。结论:小鼠脾脏Foxp3变化过程为先下降后升高;Treg在酵母多糖引起的炎症反应中起着重要的作用。 Objective： To investigate the effect of Foxp3 on inflammatory response induced by Zymosan by study the changes of mouse symptoms and the changes of Foxp3 in splenocyres. Methods： Immtmofluorescence and Real-time PCR are used to investigate the expression of Foxp3 in spleen. Results： The poisoning symptoms occured at 6h, the symptoms became the heaviest at ld and 2d with the highest mortality, Foxp3 in spleen started to reduce at 6h, reaching the least at 3d, and it began to increase at 5d. Conclusions： The change process of Foxp3 decreased firstly and then increased. This study indicated that Treg may play an important role in zymosan induced inflammatory response.
作者 曹丽 杨双文 贾文元 王世全 王丛丛 李丽 何二涛 汪晨
机构地区 第四军医大学西京医院麻醉科
出处 《现代生物医学进展》 CAS 2012年第5期846-848,共3页 Progress in Modern Biomedicine
基金 国家自然科学基金项目(30972854)
关键词 酵母多糖 SIRS 调节性T细胞 转录因子FOXP3 Zymosan SIRS Regulatory T cell Transcription factor Foxp
分类号 R392.12 [医药卫生—免疫学]
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参考文献16

  • 1Ono M,Yaguchi H,Ohkura N,et al.Foxp3 controls regulatory T-cellfunction by interacting with AML1/Runx1[J].Nature,2007,446:685-689.
  • 2Johnson SB,Lissauer M,Bochicchio GV,et al.Gene expression profi-les differentiate between sterile SIRS and early sepsis[J].Ann.Surg,2007,245,611-621.
  • 3Monneret G,Debard AL,Venet F,et al.Marked elevation of humancirculating CD4+CD25+regulatory T cells in sepsis-induced immuno-paralysis[J].Crit.Care Med,2003,31,2068-2071.
  • 4Sakaguchi S,Regulatory T.cells:Key controllers of immunologic self-tolerance[J].Cell,2000,101,455-458.
  • 5Chen W,Jin W,Hardegen NG,et al.Conversion of peripheral CD4+C-D25-naive T cells to CD4+CD25+regulatory T cells by TGF-beta ind-uction of transcriptionfactor Foxp3[J].J.Exp.Med,2003,198:1875-1886.
  • 6Wing K,Sakaguchi,S.Regulatory T cells exert checks and balanceson self tolerance and autoimmunity[J].Nat.Immunol,2010,11:7-13.
  • 7Belkaid Y,Rouse BT.Natural regulatory T cells in infectious disease[J].Nat.Immunol,2005,6,353-360.
  • 8Bone RC,Balk RA,Cerra FB.American College of Chest Physicians/Society of Critical Care Medicine Consensus Conference Definitionsfor sepsis and organ failure and guidelines for the use of innovative th-erapies in sepsis[J].Crit.Care Med,1992,20,864-874.
  • 9Shayevitz JR,Miller C,Johnson KJ,et al.Multiple organ dysfunctionsyndrome:end organ and systemic inflammatory response in a mousemodel of nonsepti corigin[J].Shock,1995,4:389-396.
  • 10Ono M,Yaguchi H,Ohkura N,et al.Foxp3 controls regulatory T-cellfunction by interacting with AML1/Runx1[J].Nature,2007.,446:685-689.

同被引文献16

  • 1Seely A J, Christou N V. Multiple organ dysfunction syn- drome: exploring the paradigm of complex nonlinear sys- tems[J]. Crit Care Med, 2000,28(7):2193-2200.
  • 2Diao L, Marshall A H, Dai X, et al. Burn plus lipopo- lysaccharide augments endoplasmic reticulum stress and NLRP3 inflammasome activation and reduces PGC-lct in liver[ J ]. Shock, 2014,41 (2) : 138-144.
  • 3Kraft R, Herndon D N, Finnerty C C, et al. Occurrence of muhiorgan dysfunction in pediatric burn patients: incidence and clinical outcome[J]. Ann Surg, 2014,259(2) :381-387.
  • 4Wing K, Sakaguchi S. Regulatory T cells exert checks and balances on self tolerance and autoimmunity [ J ]. Nat Im- munol, 2010,11(1) :7-13.
  • 5Liesz A, Suri-Payer E, Veltkamp C, et al. Regulatory T cells are key cerebroprotective immunomodulators in acute experimental stroke[J]. Nat Med, 2009,15(2):192-199.
  • 6Mikkelsen S R, Long J M, Zhang L, et al. Partial regula- tory T cell depletion prior to acute feline immunodeficiency virus infection does not alter disease pathogenesis [ J]. PLoS One, 2011 ,6(2) :e17183.
  • 7Liu Q Y, Yao Y M, Yu Y, et al. Astragalus polysaccha- rides attenuate postburn sepsis via inhibiting negative im- munoregulation of CD4 + CD25 (high) T cells [ J ]. PLoS One, 2011,6(6) :e19811.
  • 8Hein F, Massin F, Cravoisy-Popovic A, et al. The rela- tionship between CD4+ CD25 CD127-regulatory T cells and inflammatory response and outcome during shock states[J]. Crit Care, 2010,14(1) :R19.
  • 9Jia W, Cao L, Yang S, et al. Regulatory T cells are protec- tive in systemic inflammation response syndrome induced by zymosan in miee[J]. PLoS One, 2013,8(5):e64397.
  • 10Jansen M J, I-Iendriks T, de Man B M, et al. Interleukin 10 mitigates the development of the zymosan-induced multi- ple organ dysfunction syndrome in mice [ J ]. Cytokine, 1999,11 (9) :715-791.

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现代生物医学进展
2012年 第5期

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