骨桥蛋白在胰腺癌中的表达及其临床意义

Expression of Osteopontin in Pancreatic Cancer and Its Clinical Significance

目的:研究骨桥蛋白OPN在胰腺癌中的表达水平及其与临床病理特征的关系。方法:采用RT-PCR和免疫组织化学方法分别检测50例胰腺癌手术切除标本和20例癌旁正常胰腺组织中OPNmRNA及其蛋白水平的表达。结果:胰腺癌组织中OPNmRNA高表达率为90%(45/50)明显高于其在癌旁正常胰腺组织中的表达15%(3/20),差异有统计学意义(P<0.01);OPN蛋白的阳性率为86.0%(43/50),明显高于癌旁正常胰腺组织中的表达30%(6/20),差异有统计学意义(P<0.01);OPN在胰腺癌组织中表达与其淋巴结转移明显相关(P<0.05)。结论:OPN在胰腺癌中的过表达对胰腺癌的生长、浸润及转移起重要作用。

To study the mRNA and protein expression levels of osteopontin in pancreatic cancer and the relationship with clinic pathological features. Methods： Amount of 50 cases of pancreatic cancer surgical specimens and 20 cases of adjacent normal tissue were studied. The OPN mRNA and protein expression levels were detected by RT-PCR and immunohistochemistry respectively. Results： RT-PCR analysis showed that the OPN mRNA were highly expressed in pancreatic tissues （45/50, 90 % ）, which was significantly higher than in adjacent normal tissues （3/20, 15 %） （P〈0.01）. Immunohistochemistry analysis demonstrated that the OPN protein was high expressed in pancreatic tissues （43/50, 86 %）, which was significantly higher than in adjacent normal tissue （6/20, 30 %） （P〈0.01）. The expression levels of OPN in pancreatic cancer were obviously associated with lymph node metastasis （P〈0.05）. Conclusion： The OPN was overexpressed in pancreatic cancer, which maybe played important roles in the growth, invasion and metastasis of cancer.