Borealin及CPC相关基因在胚胎干细胞畸变过程中的表达差异

The Different Expression of Borealin and Genes Related to CPC during the Tumor Progression of Human ES Cells

目的:研究Borealin及CPC(Chromosoilmal Passenger Complex)相关基因表达变化与胚胎干细胞瘤变过程的关系,从而了解Borealin在干细胞中的特殊功能。方法:培养不同状态的胚胎干细胞及畸胎癌细胞,并提取总RNA,进行芯片和荧光定量PCR分析。结果:ES(embryonic stem cell)细胞分化过程中Borealin基因表达下调;在ES细胞染色体发生变异的过程中部分染色体复合物相关基因的表达发生了明显的变化,EC(embryonic cancercell)细胞中Borealin、Survivin,MCAK、Aurora B、Op18等基因的表达明显高于正常胚胎干细胞。结论:Borealin及CPC异常的表达可能是胚胎干细胞瘤变的一个重要标记。

Objective：To study the relationship between the CPC genes and the human ES cells tumor progression and understand the function of Borealin in hESCs. Methods： Using mieroarray and realtime-PCR to detect the expression levels of CPC genes in human ES cells. Results and Conclusions ： During the tumor progression of human ES cells, expression levels of genes related to the CPC were observed. Expression of Borealin, Survivin, MCAK, Aurora B, Op18 in EC are higher than in ES cells. Borealin and Survivin may be an important marker for tumor.