防己黄芪汤对兔肺缺血再灌注损伤的保护作用

Protection of Fangjihuangqi Tang for Lung Ischemia-Reperfusion Injury in Rabbits

目的观察防己黄芪汤对兔在体肺缺血再灌注损伤的保护作用。方法日本长耳大白兔随机分为肺缺血再灌注模型组和防己黄芪汤治疗组。气管切开后利用人工呼吸机行单肺通气,左肺门阻断2h后,恢复双肺通气4h分别取回左肺下叶肺组织用于组织病理检查和免疫组织化学染色检查,计算受损肺泡百分率。结果模型组肺缺血再灌注后左肺出血加重,肺间质及广泛肺泡腔内有水肿液,受损肺泡百分率显著增加,Caspase-3表达量增加。治疗组因术前使用中药防己黄芪汤,左肺组织结构完整,无明显间质水肿,受损肺泡百分率和Caspase-3表达量明显降低(均P<0.01)。结论中药防己黄芪汤对兔肺缺血再灌注损伤有一定的保护作用。

Objective To observe the protective effects of Fangjihuangqi Tang on lung ischemia-reperfusion（I/R） injury in rabbits.Methods 28 rabbits were randomly divided into ischemia-reperfusion（I/R） group and treatment group.Rabbits were killed after when the left hilum was isolated and clamped for 2h and the lungs were ventilated for another 4h.Fangjihuangqi Tang（2 mL/kg） were fed before operation.Lung pathomorphologic and immunotype changes were observed and the percentage of damage alveoli was measured.Result In I/R group after reperfusion,the left lung became diffusely hemorrhagic and produced interstitial or alveolar edema.The percentage of elamage alveoli in the left lung after reperfusion was significanthy high than after ischemia（P〈0.01）.The alveolar epithelial cell overexpressed Caspase-3.Conclusion Fangjihuangqi Tang has a protective effect for the experimental rabbit lung ischemia/reperfusion injury.