两种棉花防御反应次生代谢物质及其对烟粉虱羧酸酯酶活性的影响

Two secondary defensive cotton metabolites and their effects on the specific activities of carboxylesterase in Bemisia tabaci

通过烟粉虱Bemisia tabaci(Gennadius)取食为害诱导棉花防御反应次生代谢物质槲皮素和蛋白酶抑制剂的作用,以及2种次生物质对烟粉虱主要解毒酶羧酸酯酶的诱导表达作用,初步探索棉花防御与害虫反防御策略。结果表明烟粉虱取食对棉叶内槲皮素和蛋白酶抑制剂的诱导表达迅速但短暂,取食后1～3d,对槲皮素的最大诱导表达量为2.09mg/g,对蛋白酶抑制剂的最大诱导表达量为1.85U。同时槲皮素和蛋白酶抑制剂2种植物次生代谢物质在0.005%至1%浓度范围内,均能够诱导烟粉虱体内羧酸酯酶比活力过量表达。在浓度为0.1%时,羧酸酯酶的比活力达到最大值,分别为0.0725OD/30min和0.07825OD/30min;2个处理的剂量效应趋势非常相似。

The effects of two kinds of secondary cotton metabolites,quercetin and protease inhibitor,on carboxylesterase activity in Bemisia tabaci （Gennadius） were investigated.Results suggest that both quercetin and protease inhibitor were induced rapidly by the sucking of B.tabaci;quercetin levels reached 2.09 mg/g and protease inhibitor 1.85 units after 3 d of feeding by B.tabaci.The specific activities of carboxylesterase were increasingly induced by concentrations of quercetin and protease inhibitor of 0.005%-1%;maximum values obtained at 0.1% concentration of these compounds were 0.0725 OD/30min and 0.07825 OD/30min,respectively.These trends were quite similar for both compounds.