健脾止动汤对Tourette综合征模型鼠纹状体多巴胺转运蛋白表达的影响

Effects of Jianpizhidong decoction on dopamine transporter mRNA in striatum of tourette＇ syndrome model mice

目的研究健脾止动汤对多发性抽动症（Ts）模型鼠纹状体多巴胺转运蛋白（DAT）表达的影响。方法32只雄性ICR小鼠分成4组，即空白组、模型组、泰必利组、健脾止动汤组。除空白组外，其余3组小鼠腹腔注射亚氨基二丙腈建立Ts小鼠模型。除空白组和模型组给予生理盐水外，其余2组分别灌胃给予相应药物。4周后采用博士德公司的小鼠DATmRNA原位杂交试剂盒检测小鼠脑组织DATmR-NA的表达和分布。结果Ts模型鼠表现出抽动症特征性的运动行为和刻板行为。模型组、泰必利组以及中药组小鼠纹状体部DATmRNA的表达[分别为（0．17±0．017），（0．20±0．021），（0．24±0．022）]分别比空白对照组（0．30±0．036）减少13％、10％和16％。结论健脾止动汤治疗小儿多发性抽动症的作用可能是通过上调DAT基因表达，进而抑制多巴胺神经元功能亢进，达到抗抽动的效果。

Objective To study the effects of Jianpizhidong decoction on dopamine transporter （DAT） mRNA in tourette syndrome （TS） model mice striatum, and provide neural biochemical proof for Jianpizhidong decoction in treating children with TS. Methods 32 male ICR mice were randomly divided into blank control group, model group, Tiapride group ,Jianpizhidong decoction group. Except blank control group, the other 3 groups mice were made to TS models by intraperitoneal injection of 3,3 ＂-iminodipropionitrile. The blank and model groups, other 2 groups were given corresponding drugs by intragastric administration respectively. Results The striatum DA TmRNA expression of TS model group, Tiapride group and Tianpizhidong decoction group （respectively （0.17±0. 017）, （0.20 ±0.021 ）, （0.24 ±0. 022） ） decreased than that（0.30 ± 0. 036） of blank control group 13% , 10% and 16% respectively. Conclusion The function of improving symptoms of TS by Jianpizhidongdecoction may be relate to raised DAT gene expression to control the excessive hyperfunction of the DA neurons, and it maybe have better therapeutic than Tiapride.