摘要
目的探讨CDNF对6-羟多巴胺(6-OHDA)诱导的细胞凋亡效应的抑制作用。方法应用Li-po2000将重组真核表达质粒pDsRed-C1-CDNF转染至BMSCs,经G418筛选后获得稳定表达CDNF的MSCs细胞株(重命名为CDNF-MSCs细胞株)。应用MTT法和AnnexinⅤ-FITC细胞凋亡分析法分别检测CDNF-MSCs细胞上清液对6-OHDA诱导的PC12细胞凋亡作用的影响。结果通过G418筛选成功获得稳定表达CDNF的MSCs转染细胞,CDNF-MSCs细胞上清液预处理的PC12细胞对6-OHDA诱导的凋亡效应具有抑制作用,能有效的降低细胞的凋亡率。结论 CDNF作为一种分泌性蛋白,可显著抑制6-OHDA诱导PC12细胞凋亡。
Objective To discuss the inhibition of CDNF in 6-OHDA-induced apoptosis effect.Methods The CDNF been transfected to MSCs by Lip2000.After the screening by G418,MSCs will be stabilize expression CDNF(also calls CDNF-MSCs cells).Through the MTT analysis and Annexin Ⅴ-FITC cell apoptosis analysis detects the supernatant to 6-OHDA induction of PC12 cell apoptosis.Results Through the screening by G418,MSCs transfected cells will stably express CDNF.The supernatant of CDNF-MSCs which have a inhibition effect for it pretreatment of PC12 cells,can effectively reduce the rate of apoptosis.Conclusion CDNF which is a secreted protein will striking reduce the 6-OHDA-damaged PC12 cells.
出处
《立体定向和功能性神经外科杂志》
2012年第1期5-8,共4页
Chinese Journal of Stereotactic and Functional Neurosurgery