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CDNF基因修饰的大鼠骨髓间充质干细胞对6-OHDA诱导PC12细胞凋亡的抑制作用 被引量：2

Expressions of CDNF in rat bone marrow stromal cells and its neuroprotection for the PC12 cells damaged by 6-OHDA
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摘要 目的探讨CDNF对6-羟多巴胺(6-OHDA)诱导的细胞凋亡效应的抑制作用。方法应用Li-po2000将重组真核表达质粒pDsRed-C1-CDNF转染至BMSCs,经G418筛选后获得稳定表达CDNF的MSCs细胞株(重命名为CDNF-MSCs细胞株)。应用MTT法和AnnexinⅤ-FITC细胞凋亡分析法分别检测CDNF-MSCs细胞上清液对6-OHDA诱导的PC12细胞凋亡作用的影响。结果通过G418筛选成功获得稳定表达CDNF的MSCs转染细胞,CDNF-MSCs细胞上清液预处理的PC12细胞对6-OHDA诱导的凋亡效应具有抑制作用,能有效的降低细胞的凋亡率。结论 CDNF作为一种分泌性蛋白,可显著抑制6-OHDA诱导PC12细胞凋亡。 Objective To discuss the inhibition of CDNF in 6-OHDA-induced apoptosis effect.Methods The CDNF been transfected to MSCs by Lip2000.After the screening by G418,MSCs will be stabilize expression CDNF（also calls CDNF-MSCs cells）.Through the MTT analysis and Annexin Ⅴ-FITC cell apoptosis analysis detects the supernatant to 6-OHDA induction of PC12 cell apoptosis.Results Through the screening by G418,MSCs transfected cells will stably express CDNF.The supernatant of CDNF-MSCs which have a inhibition effect for it pretreatment of PC12 cells,can effectively reduce the rate of apoptosis.Conclusion CDNF which is a secreted protein will striking reduce the 6-OHDA-damaged PC12 cells.
作者 韩暄 张俊 牛朝诗
机构地区 安徽医科大学附属省立医院神经外科安徽省脑立体定向神经外科研究所
出处 《立体定向和功能性神经外科杂志》 2012年第1期5-8,共4页 Chinese Journal of Stereotactic and Functional Neurosurgery
关键词 帕金森病 骨髓间充质干细胞 脑多巴胺能神经营养因子 Parkinson＇s disease Bone marrow stromal cells Cerebral dopamine neurotrophic factor
分类号 R742.5 [医药卫生—神经病学与精神病学]
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参考文献9

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二级参考文献12

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共引文献5

同被引文献22

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立体定向和功能性神经外科杂志
2012年 第1期

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