不同成熟期“北春”越橘花色苷含量及抗氧化研究

Study on content and antioxidant activity of anthocyanins from different maturity of Northcountry bilberry fruits

为充分开发利用越橘属植物资源,确定适宜采收期,分别采收绿白期、粉熟期、亮蓝期、后熟期的越橘栽培品种"北春",对四个不同成熟期的果实进行花色苷含量及体外抗氧化活性研究,并对越橘果实中糖酸含量与花色苷含量进行了相关性分析。结果表明,随着果实的成熟,花色苷含量逐渐增加,且在亮蓝期达到最高150.54mg/100g;果实的花色苷含量与糖酸比呈正相关;4个不同成熟期的"北春"果实花色苷均有良好的抗氧化活性,且花色苷的抗氧化活性与其含量呈正相关,亮蓝期"北春"越橘的还原能力、抗脂质过氧化能力、清除羟自由基能力、清除超氧阴离子自由基能力和清除DPPH自由基能力均最高,且其抗脂质过氧化能力、清除羟自由基的能力均高于抗坏血酸。

To take full advantage of the bilberry resources and determine appropriate harvest time,four different maturity stage：green white period,powder ripe period,bright blue period,and mature late period of cultivated variety Northcountry bilberry were harvested respectively.Then the content and antioxidant activity of anthocyanins were studied and found that the ratio of sugar to acid could affect the content of anthocyanins.The results showed that the correlation between the content of anthocyanine and the ratio of sugar to acid were always positive,and the increase of sugar-acid ratio also promoted the formation of anthocyanins;with the maturity of the fruits,anthocyanins content increased gradually,and in bright blue period reached the highest 150.54mg in 100g fruit.The anthocyanin of all those four harvest times of Northcountry fruits had excellent antioxidant activities,and the anthocyanins content was positively correlated with the antioxidant activity.In Northcountry bilberry bright blue period,the antioxidant activity such as DPPH free radical-scavenging power assay,reducing power assay and their scavenging power assay on lipid peroxidation,·OH and O2-· were highest.The scavenging power assay on lipid peroxidation and ·OH of cultivated bilberry variety Northcountry fruits was higher than VC.