孕鼠与非孕鼠重症急性胰腺炎多器官损伤的对比

Comparative experimental study on multiple organ damage between pregnant rats and non-pregnant rats with severe acute pancreatitis

目的观察孕鼠和非孕鼠重症急性胰腺炎（SAP）各器官的功能和组织病理变化。方法SD孕鼠分为孕鼠组和孕鼠SAP组，非孕雌鼠分为SO组和SAP组，分别设立3、6、12h时间点，各时间点8只。检测血清淀粉酶（AMY）、肝功能酶学指标[丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）]、肾功能[肌酐（Cr）、尿素氮（BUN）]，观察胰腺、肝脏、肾脏、肺和心脏组织病理变化。结果孕鼠SAP组AMY和胰腺组织病理评分在3、6h显著高于SAP组（P〈0．05），肝、肾功能在3．6、12h均高于SAP组[3h AIJT（128．38±24．92）比（99．75±16．27）U／L，AST（261．25±64．98）比（194．50±49．00）U／L，Cr（52．38±8．99）比（41．88±7．28）μmol／L，BUN（8．63±1．24）比（7．11±1．29）mmol／L，P〈0．05]，肝脏和肾脏组织病理评分（6h肝脏2．13±0．44比1．56±0．49，肾脏5．25±1．28比3．88±1．13），在6、12h，肺脏组织病理评分在12h显著高于SAP组（5．00±0．85比3．75±0．49，P〈0．05）。结论孕鼠较非孕鼠胰腺炎发病要急且病情进展更快，并发多器官损伤时间更早且损伤程度更重。

Objective To observe the functional changes and the histopathological changes of mul- tiple organs in pregnant rats and non-pregnant female rats with severe acute pancreatitis （SAP）. Methods Pregnant SD rats were randomly divided into pregnancy group and SAP group, and non-pregnant female rats were randomly divided into sham operation group （SO group） and SAP group. Rats were killed at 3rd, 6th and 12th h. Serum amylase （AMY）, liver function [ alanine transaminase （ALT）, aspartate aminotrans- ferase （AST）], and renal function [creatinine （Cr）, blood urea nitrogen （BUN）] were detected. The pathological changes in the pancreas, liver, kidney, lung and heart tissues were observed. Results Amylase and histological scores of the pancreas at 3rd, and 6th h were higher significantly in SAP group of pregnant rats than those in SAP group （P 〈 0. 05）. Liver function levels at each time point were higher significandy in SAP group of pregnant rats than those in SAP group [3 h： ALT （ 128.38±24. 92） vs （99.75±16.27） U/L, AST （261.25±64.98） vs （194.50±49.00） U/L, Cr （52.38 ±8.99） vs （41.8±7. 28 ） Ixmol/L, BUN （ 8. 63±1.24 ） vs （ 7.11 ±1.29 ） mmol/L, P 〈 0.05 ]. Histological scores of the liver and kidneys at 3rd and 6th h （6 h： liver 2. 13 ±0. 44 vs 1.56±0.49, kidney 5.25±1.28 vs 3.88±1.13 ,P 〈0. 05）, and those of the lungs at 12th h （5.00±0. 85 vs 3. 75 ±0. 49,P 〈0. 05） were higher significantly in SAP group of pregnant rats than in SAP group. Conclusion Compared with non-pregnant rats, the pancreatitis occurring in pregnant rats is more acute and more severe, and multiple organ damage occurs earlier and more severe.