摘要
对杜泊绵羊、小尾寒羊和晋中绵羊3个品种的MSTN基因编码序列进行克隆测序,并比较分析该基因编码区的SNPs。结果表明:在引物P1、P2、P3扩增的MSTN基因片段中存在10个SNPs,其中4个SNPs位于非编码区,6个SNPs位于编码区;编码区中有2个SNPs为无义突变,其他4个为错义突变。因此,绵羊MSTN基因在进化过程中保守性不强,可能存在较多的变异位点,为进一步群体遗传分析提供了基础。
In this study,the SNPs in the MSTN gene of Jinzhong sheep,Small-tailed Han sheep and Dorper sheep were analysed by DNA sequencing.The results showed that 10 SNPs were found in the sequences amplified using primer pair P1,P2,and P3,and four SNPs were in noncoding region,the other six in coding region.In the coding regions,two SNPs were non-sense mutations and the other 4 SNPs were mis-sense mutations.Thus,it can be concluded that the sheep MSTN gene is not highly conserved and may has more mutations,which can provide a basis for further analysis of population genetics.
出处
《中国草食动物》
2012年第2期12-15,共4页
China Herbivores
基金
山西省自然科学基金资助项目(2010011045)
