雌激素诱导子宫内膜癌细胞血管因子生成的作用

Effect of estrogen on growth of endometrial cancer cells and expression of angiogenic factors

目的探讨雌激素诱导子宫内膜癌细胞HEC-1A血管因子生成的机制,阐述雌激素在子宫内膜癌发生中的作用。方法以雌激素(E2组)作用于HEC-1A细胞30min,雌激素受体抑制剂(ER组)、AKT抑制剂(AKT组)、NF-κB抑制剂(NF-κB组)分别预处理HEC-1A细胞1h,各加入雌激素作用30min后;采用qRT-PCR检测各组细胞内血管因子mRNA表达,Westernblot检测血管因子蛋白的表达情况。结果 E2组的血管因子mRNA及蛋白的表达均明显高于对照组(P<0.05);ER组、AKT组及NF-κB组的血管因子mRNA和蛋白的表达均明显低于E2组(P<0.05)。结论雌激素在子宫内膜癌HEC-1A细胞中起到信号传导的作用,可调节血管生成因子的表达,参与子宫内膜癌发生、发展的过程。

Objective To explore the role of estrogen in endometrial cancer by examining the expression of angiogenic factors in the estrogen-induced endometrial cell line HEC-1A.Method Real-time quantitative PCR and Western blots were used to analyze the mRNA and protein expression of angiogenic factors in HEC-1A cells after stimulation with estrogen for 30 min with or without 60-min pretreatment with inhibitors against the estrogen receptor,the AKT kinase pathway,and the NF-κB pathway.Result Real-time PCR and Western blots showed that mRNA and protein expression of angiogenic factors were significantly higher in the estrogen-stimulated group than in the unstimulated control group（P0.05）.Pretreatment with signaling pathway inhibitors significantly reduced mRNA and protein expression of angiogenic factors below the levels in the estrogen-stimulated group that was not pretreated（P0.05）.Con-clusion Estrogen induces the production of angiogenic factors by activating signal transduction pathways.