英连金化浊解毒方对慢性萎缩性胃炎癌前病变大鼠PG-ⅠBigET-1、VEGF表达的影响

Effect of Yinglianjin Huazhuo Detoxification Fang on PG-I BigET-1 VEGF's Expression of the Precancerous Lesions Gastric Cancer's Rat

目的:观察英连金化浊解毒方对慢性萎缩性胃炎癌前病变大鼠的作用机理研究。方法:将120只大鼠随机分为空白组、模型组、中药大、中、小剂量组及胃复春组。采用N-甲基-N-硝基N-亚硝基胍(MNNG)溶液自由饮用,乙醇溶液灌胃,配合饥饱失常等综合方法诱导造模12周。造模成功后分组治疗,第24周后处死全部实验大鼠进行TUNEL细胞凋亡检测以及胃蛋白酶原Ⅰ(PG-Ⅰ)、内皮素(Big ET-1)、血管内皮细胞生长因子(VEGF)等指标检测观察其改变情况。结果:空白组、中药治疗组、胃复春组与模型组比较血浆Big ET-1含量明显降低,胃黏膜组织VEGF阳性率明显降低,血清PG-Ⅰ含量明显升高,差异均有统计学意义(P<0.05)。结论:英连金化浊解毒方能够降低癌前病变大鼠血浆Big ET-1含量及胃黏膜VEGF蛋白的表达率,提高血清PG-Ⅰ含量,恢复正常胃黏膜及腺体细胞的功能。

Objective： Observe the Yinglianjin huazhuo detoxification fang treatment on the Chronic atrophic gastritis precancerous lesions rats the mechanism. Methods ： 120 rats were randomly divided into blank group, model group, large, medium and small dose group and Weifuchun group. By N-methyl-N- nitro-N-nitrosoguanidine （ MNNG ） solution free drink, ethanol garage, with irregular diet induced such an integrated approach to modeling for 12 weeks. After the modeling, the treatment group, 24 weeks later all rats were TUNEL detection of apoptosis and pepsinogen I （ PG- Ⅰ ）, endothelin （ Big ET-1 ）, vascular endothelial growth factor （ VEGF ） Detection and other indicators to change the situation observed. Resuhs ： The control group, treatment group, Weifuchun compared with model group, levels of plasma Big ET-1 significantly decreased VEGF-positive gastric mueosa was significantly decreased, serum PG-Ⅰ was significantly increased, the differences were statistically significant （ P〈O.05 ） .Conclusion ： The Yinglianjin huazhuo detoxification fang reduce precancerous lesions of rats with better Big ET-1 levels in plasma and gastric mucosal expression of VEGF protein, increase levels of serum PG- Ⅰ, inhibit excessive cell proliferation and induced apoptosis, normal gastric mueosa and glandular cell function, and thus play a role in the treatment of gastric precancerous lesions.