摘要
目的探讨自主研制的志贺氏菌显色培养基的应用效果。方法采用30株标准菌株及50份自然样品检测试验,比对自主研制的志贺氏菌显色培养基、梅里埃(R&F)志贺氏菌显色培养基、木糖-赖氨酸-去氧胆酸盐培养基(XLD培养基)和麦康凯琼脂培养基的选择性。结果自主研制的志贺氏菌显色培养基能有效分离志贺氏菌属菌株,50份自然样品的检出率与R&F志贺氏菌显色培养基相同,均达到10%。结论自主研制的志贺氏菌显色培养基与R&F志贺氏菌显色培养基在特异性及检出率达到一致。
Objective To explore the application effect of the shigella chromogenic medium based on independent innovation.Methods A total of 30 standard strains were used to test the chromogenic medium,and 50 food samples on sale were detected by shigella chromogenic medium based on independent innovation,RF shigella chromogenic medium, XLD and MacConkey agar.Results Shigella spp.could be effectively detected by Shigella chromogenic medium based on independent innovation,the detection rate of food samples was 10%by innovative products and RF Shigella chromogenic medium.Conclusion The specialty,sensitivity and detection rate of innovative shigella chromogenic medium and RF shigella chromogenic medium are consistency.
出处
《中国国境卫生检疫杂志》
CAS
2012年第1期6-8,26,共4页
Chinese Journal of Frontier Health and Quarantine
基金
国家质检总局科研基金项目(2010IK173)
关键词
志贺氏菌
特异性
显色培养基
自主研制
Shigella
Specificity
Chromogenic medium
Independent innovation