定量PCR技术检测正畸患者牙龈卟啉单胞菌的变化

Real-Time quantitative PCR for porphyromonas gingivalis in orthodontic patients

目的采用荧光实时定量PCR技术，研究正畸患者的龈下菌斑中牙龈卟啉单胞菌（Porphyromonas gingivalis，Pg）在总细菌中的构成比是否产生变化。方法挑选30例正畸治疗六个月以上患者为实验组，30例未带矫治器的牙周健康者为对照组，分别记录牙周临床指标（包括菌斑指数、龈沟出血指数、探诊深度），和龈下菌斑中牙龈卟啉单胞菌在总细菌中的构成比，并比较两组患者的差异。结果菌斑指数实验组为1．60±0．58，对照组为1．09±0．72，两组比较差异有统计学意义（P〈0．05）。探诊深度实验组为（2．72±0．64）mm，对照组为（2．13±0．18）mm，两组比较差异有极显著的统计学意义（P〈0．001）。龈沟出血指数实验组为1．4±0．47，对照组为1．32±0．52，两组间比较差异无统计学意义（P=0．78）。牙龈卟啉单胞菌在总细菌中的构成比，实验组0．45％±0．07％和对照组0．05％±0．01％比较，P〈0．0001，差异有统计学意义。结论正畸患者龈下菌斑中，牙龈卟啉单胞菌在总细菌中的含量增加，荧光实时定量PCR技术可监测其变化，提示正畸期间保持牙周清洁的重要性。

Objective Using real-time quantitative polymerase chain reaction （PCR） in orthodontic patients, and to investigate the effect of fixed appliance on the proportion of Porphyromonas gingivalis in total bacteria in subgingival plaques. Methods 30 patients with fixed appliance over 6 months were randomly selected as experimental group, and 30 without the appliance comprised as control group. Then examine the clinical indexes （Plaque index PLI, Sulcus bleeding index SBI, Probing depth PD） and the proportion of P. gingivalis in total bacteria in subgingival plaques, then find the difference between two groups. Results The resuIts showed that there was significant difference statistically （P〈0. 05） in PLI between the experimental （1.60 ± 0. 58） and the control group （1.09±0. 72）. There was significant difference statistically （P〈0. 001） in PD between the experimental （2.72±0.64） mm and the control group （2. 13±0. 18） mm SBI had no significant difference （P=0. 78） between two groups. And the proportion of P. gingivalis in total bacteria in subgingival plaques, in experimental group （0. 4%±0. 07%） were more significant than that in control group （0. 05%± 0. 01%） （P〈0. 0001）. Conclusions Fixed orthodontic appliances caused the increasement of the proportion of P. gingivalis in total bacterias in orthodontic patients, we can find the variation through real-time quantitative polymerase chain reaction.