乙型肝炎患者外周血单个核细胞和血清中HBV-DNA含量与HBVM间关系的探讨

The relationship discuss between HBV - DNA and HBVM in hepatitis B patients PBMC and serum

目的探讨乙肝患者外周血单个核细胞和血清中HBV-DNA含量及其与HBVM之间的关系。方法67例乙肝患者为检测对象，36健康献血者为对照组，分别用FQ-PCR定量测定，内对照法定量测定血清HBV-DNA。免疫荧光定量测定HBVM。结果外周血单个核细胞HBV-DNA检出阳性率为67．2％（45／67），与血清的符合率为80．7％，但慢性乙肝患者外周血单个核细胞HBV-DNA检出率高于血清，且与病情呈正相关。结论三个指标的联合检测有助与反映肝细胞病毒复制情况。对进一步指导抗病毒治疗有一定意义。

Objective To investigate the relationship between the appearance of HBV - DNA in Peripheral Blood Mononuclear Cells （PBMC）, the quantity of serum in hepatitis B（HBV） patients and patterns of HBVM of HBV. Methods 67 patients with hepatitis B were selected as study objects and 20 blood samples from blood station were collected as control group. HBV loads of the patients were assayed by real - time fluorescent quantitative PCR（RTFQ- PCR） in PBMC, We constructed inner control to deteete quantitative PCR in serum, and HBV serologic markers of the patients were detected by fluoroimmunoassay instrument. Conclusions Among 62eases of HBV patients, the total posi- tive rates of HBV - DNA in PBMC was 67.2% （45/67）, and 80.7% results accorded with those in serum. But the total positive rates of HBV - DNA in PBMC was obviously higher than in serum in ehronie hepatitis B, and relationship with grave degree of the patient＇ s condition. Resuits The eomblnafion of multiple methods has supplementary effeets, and reflected the virus replication indirectly, and also beneficial to the anti - virus therapy.