多种动物血清中生物活性LH／CG的放射受体测定法（RRA）

Establishment of a Radioreceptor Assay(RRA) Capable of Measuring Serum Bioactive LH/CG in a Wide Variety of Species

利用绵羊卵巢黄体细胞膜作为ＬＨ/ＣＧ受体来源，以放射性＾１２５Ｉ－hＣＧ作为示踪标记配体，以高纯度的hＣＧ为标准品建立标准曲组，建立了一种有效的ＬＨ－ＲＲＡ测定方 法。它能够址接检测多种动物血清中与受体结合的具有生物活性的ＬＨ的动态变化。该法测定程序简单，在２h内完成并获得可重复性结果。测定方法的灵敏度为０．３ng/管，批内和批间误差分别为（３．４１±３．１４）％和（８．７０±７．９３）％。动物（牛、兔、

An LH radioreceptor assay (RRA) which allows direct measurement of receptor bindable bioactive serum LH in a variety of species was developed and validated. Using sheep luteal membrane as the receptor preparation source and 125 I labelled hCG as the tracer radioligand, the standard curve was obtained using high purified hCG. The assay is simple and can be completed within 2 hours. The sensitivity of the assay was 0.3 ng LH/per tube correspondent to hCG activity. The intra and interassay variations were (3.41±3.14)% and (8.70±7.93)%, respectively. Both the dose response curve of pituitary extract from bovine, swine, rabbit and the serum from bovine, ovine, swine, rabbit, rat, hamster showed parallelism to the hCG standard curve indicating that the RRA can be used to monitor the kinetic change of serum LH in a variety of species. The recoverable rates of 3, 10 and 30 ng hCG added to the diluted ram serum were (100.59±5.55)%, (99.55±9.86)% and (104.28±7.45)%, respectively, which demonstrated the veracity of the RRA results. The level of LH measured correlated with the physiological status of the animals. The bioactive LH level in pituitary extract was by far higher than that in circulation.