水浴湿法消化Zeeman石墨炉原子吸收法测定肉鸡肝组织中硒含量

Determination of Selenium Content in Broiler Liver with Wet Digestion in Boiling Water Bath by Zeeman Graphite Furnace Atomic Absorption Spectrometry

采用水浴湿法消化，以Ｐd（ＮＯ３）２－ＴritonＸ－１００作为基体改进剂，应用Ｚeeman石墨炉原子吸收法测定了肝组织硒含量。试验结果表明，肝组织经水浴湿法消化测得的硒 含量与消化测定结果基本一致（Ｐ〉０．０５），相对标准偏差为１．１１％，回收率为９５．８０％～１００．４０％。石墨炉原子吸收法测定硒的检测限为５．０ug/Ｌ。

The broiler liver was digested by the nitric/perchloric acids in boiling water bath and the selenium was determined by Zeeman graphite furnace atomic absorption spectrometry (GFAAS) after addition of Pd(NO 3) 2 Triton X 100 as a matrix modifer. The results demonstrated that the selenium contents in the liver digested with the acids in boiling water bath were not different from those in heating block (P>0.05) with the relative standard deviation and recovery rate to be 1.11% and 95.80% 100.40% respectively, and the detection limit of selenium for GFAAS to be 5.0 μg/L.