野菊花中性多糖CIP-C的分离纯化及结构解析

Isolation and Structural Characterization of a New Neutral Polysaccharide from Chrysanthemum indicum L.

以野菊花为原材料,经热水提取、乙醇沉淀、DEAE-Sepharose Fast Flow和Sephacryl S-200凝胶柱层析分离纯化,得到1个水溶性的中性多糖CIP-C.采用GC-MS、部分酸水解及甲基化分析等对该多糖的结构进行了解析.结果表明,该多糖主要由D-Man,D-Glc和D-Gal组成,并含有少量的D-Fuc,L-Ara和D-Xyl,其主链由β(或α)-D-1,4-Man,β-D-1,6-Glc和β-D-1,4-Gal组成,而阿拉伯糖通过α-L-T-Araf和α-L-1,5-Araf连接形成阿拉伯聚糖支链或与β-D-1,4-Galp的O3位相连形成阿拉伯半乳聚糖支链.

Chrysanthemum indicum L.was used as a heat-clearing and detoxication herb in traditional Chinese medicine.The studies indicated that the polysaccharide played a critical role in its medical effects,but there was no report about the details of the structural features of the homogenous polysacchatide from Chrysanthemum indicum L.In order to identify the structural features for the quality control,systematical studies of Chrysanthemum indicum L.were performed.A water soluble neutral polysaccharide（CIP-C） was obtained from Chrysanthemum indicum L.by hot water extraction,precipitation with ethanol,separation and purification through DEAE-Sepharose Fast Flow ion exchange chromatography and Sephacryl S-200 gel permeation chromatograph.By means of GC-MS,NMR,partially acid hydrolysis and methylation reaction,the structure of CIP-C was identified.The results show that CIP-C is a heteropolysaccharide,which is mainly composed of D-Man,D-Glc and D-Gal,with a small quantity of D-Fuc,L-Ara and D-Xyl.The backbone of CIP-C is linked by β（or α）-D-1,4-Man,β-D-1,6-Glc and β-D-1,4-Gal.In addition,T-Araf,1,5-Araf and T-Gal,1,4-Gal,1,3,6-Gal,1,3,4,6-Gal may be linked as an arabinan branch and an AGI arabinogalactan branch.In this paper,CIP-C was firstly reported as a new neutral branched polysaccharide from Chrysanthemum indicum L.