黑素瘤抑制蛋白在转基因小鼠胚胎乳腺组织中的表达

Expression of melanoma inhibitory protein in mammary buds of transgenic mice

目的：检测黑素瘤抑制蛋白（ＭＩＡ／ＣＤ－ＲＡＰ）在小鼠胚胎乳腺组织中的表达。方法：剖腹分离出１０．５～１６．５ ｄ 的ＭＩＡ／ＣＤ－ＲＡＰ启动子－半乳糖苷酶（２．２ｌａｃＺ）转基因小鼠胚胎，利用Ｘ－ｇａｌ全胚胎染色观察转基因的组织和细胞染色，并以免疫组织化学染色检测内源性ＭＩＡ／ＣＤ－ＲＡＰ蛋白在胚胎乳腺组织中的表达。结果：ＭＩＡ／ＣＤ－ＲＡＰ在孕期１１．５ ｄ 的小鼠胚胎乳腺上皮细胞中开始表达，至１３．５ ｄ 表达明显增强，而后逐渐减弱，至１６．５ ｄ 消失。结论：ＭＩＡ／ＣＤ－ＲＡＰ可能在乳腺的早期发育和乳腺癌发生中起重要作用。

Objective: To investigate the expression of melanoma inhibitory protein (MIA) in mammary buds of transgenic mice. Methods: Transgenic embryos were collected from day 10.5 to 16.5 by Cesarean section. The transgene expression was detected by whole mount staining with X gal. Immunohistochemistry was carried out to detect endogenous MIA/CD RAP expression in mammary buds. Results: MIA/CD RAP expression in mammary epithelial cells began on day 11.5 p.c. and continued throughout day 15.5 p.c., with the strongest expression observed on day 13.5 p.c. Conclusion: MIA/CD RAP may play an important role in the early organogenesis of mammary gland and carcinogenesis of breast cancer.