HPLC同时测定龙血竭及其提取物中5个有效成分的含量

Simultaneous determination of five active components in resina draconis and its extract by HPLC

目的:建立HPLC同时测定龙血竭及其提取物中龙血素A,龙血素B,7,4'-二羟基黄酮,紫檀茋,白藜芦醇含量的方法。方法:采用Kromasil 100-5 C18(4.6 mm×250 mm,5μm)色谱柱;流动相乙腈-1%冰醋酸,梯度洗脱;流速1 mL.min-1;柱温40℃;检测波长龙血素A和龙血素B为278 nm,7,4'-二羟基黄酮、紫檀茋和白藜芦醇为319 nm。结果:5个有效成分在40 min内均达到基线分离,线性关系良好(r≥0.999 7)。龙血竭中龙血素A,龙血素B,7,4'-二羟基黄酮,紫檀茋,白藜芦醇的平均回收率分别为102.9%,96.81%,97.29%,100.7%,103.7%,RSD分别为0.23%,1.5%,0.42%,0.58%,0.34%;龙血竭提取物中龙血素A,龙血素B,7,4'-二羟基黄酮,紫檀茋,白藜芦醇的平均回收率分别为102.2%,96.93%,97.90%,102.0%,103.3%,RSD分别为1.7%,0.91%,1.4%,1.5%,1.2%。结论:本方法操作简便、结果准确,具有较好的重复性和稳定性,为龙血竭及其提取物的质量控制提供了一个很好的参考。

Objective： To establish an HPLC method for simultaneous determination of contents of loureirin A,loureirin B,7,4′-dihydroxy flavone,pterostilbene and resveratrol in resina draconis and its extracts.Method： Kromasil 100-5C18 column（4.6 mm×250 mm,5 μm） was used with the mobile phase of acetonitrile-1% glacial acetic acid at a flow rate of 1.0 mL·min-1 and the column temperature at 40 ℃.The detective wave length of loureirin A and B was detected at 278 nm,and 7,4′-dihydroxy flavone,pterostilbene and resveratrol was at 319 nm.Result： All the five active components reached the resolved peaks within 40 min,indicating a good linearity（r≥0.999 7）.The average recoveries of loureirin A,loureirin B,7,4′-dihydroxy flavone,pterostilbene and resveratrol in resina draconis were 102.9%,96.81%,97.29%,100.7% and 103.7%,with RSDs of 0.23%,1.5%,0.42%,0.58% and 0.34%,respectively.The average recoveries of loureirin A,loureirin B,7,4′-dihydroxy flavone,pterostilbene and resveratrol in extract of resina draconis were 102.2%,96.93%,97.90%,102.0% and 103.3%,with RSDs of 1.7%,0.91%,1.4%,1.5% and 1.2%,respectively.Conclusion： The method is so easy,accurate,highly repeatable and stable that it provides good reference for the quality control of resina draconis and its extracts.