半夏凝集素蛋白与半夏毒针晶毒性的相关性研究

Correlation of Pinellia ternata agglutinin and Pinellia ternata raphides′ toxicity

目的:研究半夏凝集素蛋白与半夏毒针晶毒性的相关性,揭示半夏产生毒性的机制。方法:采用大鼠腹腔炎症模型,以腹腔渗出液中PGE2含量为指标,研究半夏凝集素致炎作用的量-毒、时-毒曲线;采用家兔眼结膜刺激模型,以眼结膜的病理切片观察半夏凝集素与半夏毒针晶刺激性的相关性。结果:半夏凝集素可引起大鼠腹腔渗出液中PGE2、蛋白含量显著提高,呈剂量依赖性;半夏凝集素可显著加重半夏毒针晶对家兔眼结膜的刺激性,且随着半夏凝集浓度的升高,刺激性显著增强,但单给药半夏凝集素对家兔眼结膜无刺激性。结论:半夏凝集素蛋白具强烈的致炎作用。半夏产生强烈炎症刺激的机制是半夏刺入机体,凝集素蛋白随针晶进入机体组织,诱导炎症反应发生而产生刺激性。

Objective： To study the correlation of Pinellia ternata agglutinin（PTA） and toxicity of P.ternata raphides and to find out the toxic mechanism of P.ternata.Method： PTA has obvious effect of pro-inflammation.The model of rats peritonitis was used to study the dose-toxicity and time-toxicity relationship of the effect by detecting the releases of inflammatory mediators PGE2 in the exudates.The model of Draize rabbit eye test was applied to determine the correlation of PTA and toxicity of raphides by pathological examination.Result： PTA enhanced the content of PGE2 and protein in rats peritoneal cavities concentration dependently.With PTA concentration increased,PTA enhanced the inflammation induced by raphides to rabbit eyes,but PTA alone had no toxicity response.Conclusion： PTA had obvious effect of pro-inflammation.The toxic mechanism of P.ternata was PTA induced inflammation only when the raphides pierce into the organization.