3'-甲氧基葛根素对缺血再灌注大鼠脑内氨基酸动态变化的实验研究

Study on 3′-methoxy puerarin to dynamic changes of amino acids in rat brain ischemia-reperfusion

目的:研究脑缺血时3'-甲氧基葛根素对谷氨酸、天门冬氨酸、牛磺酸和γ-氨基丁酸的抑制作用,探讨脑缺血损伤再灌注过程中其对神经损伤的保护机制。方法:制作大鼠大脑中动脉阻塞模型,利用脑微透析技术收集大鼠纹状体细胞外液中的氨基酸类神经递质,并3'-甲氧基葛根素灌胃治疗;HPLC荧光检测用药前后4种氨基酸的含量变化。结果:3'-甲氧基葛根素可降低缺血时脑组织中兴奋性氨基酸Asp,Glu的浓度,同时使抑制性氨基酸Tau,GABA含量明显降低。结论:3'-甲氧基葛根素能降低缺血引起的脑内兴奋性氨基酸的毒性,调节脑内兴奋性氨基酸的含量。因此,该药改善脑内氨基酸的变化状态是改善和保护急性期脑梗死大鼠脑内神经保护作用机制之一。

Objective： To study the effect of 3′-methoxy puerarin on cerebral ischemic glutamic acid,aspartic acid,taurine and γ-aminobutyric acid inhibitory of rats and investigate the protective mechanisms of cerebral ischemia-reperfusion.Method： Using middle cerebral artery occlusion rat model,to collect extracellular fluid in rat striatal amino acid neurotransmitters by brain microdialysis and HPLC techniques with fluorescence detection before and after 3′-methoxy puerarin treatment four kinds of amino acids changes.Result： 3′-methoxy puerarin reduced concentrations of excitatory amino acid（EAA） Asp and Glu,while Tau and GABA inhibitory amino acids were significantly reduced.Conclusion： 3′-methoxy puerarin reduce ischemia-induced brain EAA toxicity against EAA neurotoxicity,regulate the brain neurotransmitter amino acid content,improve the excitatory and inhibitory amino acid balance is one of the mechanisms that to improve and protect the important acute cerebral infarction in rat brain nuclei.