MaFGF对顺铂所致海马神经元损害保护作用的体外实验研究

Neuroprotective effect of MaFGF on hippocampal neurons damaged by cisplatin in vitro

目的探讨改构体酸性成纤维细胞生长因子(MaF-GF)对体外培养的海马神经元顺铂(DDP)损伤的保护作用。方法取新生SD乳鼠海马神经元进行原代培养,采用高分子量神经丝蛋白多克隆抗体(NF-H)进行免疫细胞化学染色鉴定。原代细胞接种于96孔培养板:①培养7 d后加入一系列浓度的DDP,继续培养24 h,采用四唑盐(MTT)比色法检测细胞存活率;②以DDP建立损伤模型,并在加DDP同时加入一系列浓度的MaFGF,观察MaFGF对海马神经元的保护作用。结果①DDP对体外培养神经元活力具有明显的抑制作用,随着DDP浓度的增加,细胞活力降低;②浓度为5.2～10.4μg.L-1的MaFGF能使DDP损伤的神经元活力明显提高;③MaFGF+DDP组和DDP组比较,各生化和酶学指标差异均有统计学意义(P<0.01),MaFGF+DDP组中超氧化物歧化酶(SOD)、谷胱苷肽过氧化物酶(GSH-Px)活性升高,丙二醇(MDA)、一氧化氮(NO)水平降低。结论MaFGF对DDP损伤的海马神经细胞具有一定的保护作用。

Aim To investigate the neuroprotective effect of MaFGF on hippocampal neurons exposed to cisplatin（DDP） in vitro.Methods Hippocampal neurons from neonatal SD rats were primarily cultured for seven days and identified by immunocytochemistry of neurofilament heavy polypeptide（NF-H）.These primary cells were inoculated into 96 well plates.① 7 days later,different concentrations of DDP were added,and MTT assay was performed to test the cell viability after 24 h.② The injury model was induced by DDP,and different concentrations of MaFGF would be added simultaneously.Finally,the protective effect was observed.Results ① The cell vitality reduced when increased the concentrations of DDP,which indicated that DDP inhibited the vigor of neurons significantly.② The survival of hippocampal neurons added with DDP increased obviously with MaFGF between 5.2 to 10.4 μg·L-1.③ There were statistical differences of biochemistry and zymology between MaFGF＋DDP group and DDP group（P0.01）.The activity of SOD and GSH-Px of MaFGF＋DDP group increased while the lever of NO and MDA decreased.Conclusion MaFGF may play a significant neuronal protective role in hippocampal neurons injured by DDP.