CD4^+CD25^+调节性T细胞在不同肝脏肿瘤中的表达及意义

Expression and Significance of CD4^+ CD25^+ Regulatory T Cells in Different Liver Tumors

目的:研究CD4+CD25+调节性T细胞(Treg)在不同肝脏肿瘤中的分布特点,评价其在肿瘤发生和发展中的作用。方法:根据病理诊断结果将80例肝脏肿瘤分为肝局灶性结节状增生(FNH)组10例、不典型腺瘤样增生(AAH)组10例以及原发性肝癌(HCC)组60例。另选取10例正常肝组织(肝血管瘤边缘肝组织)石蜡包埋标本为对照组。采用双重酶标免疫组化染色的方法测定不同肝脏肿瘤切片中Treg细胞的表达状况。对比分析Treg细胞在FNH、AAH和HCC各组中的表达特点,并进一步分析在HCC组中Treg细胞表达的影响因素。结果:对照组及FNH组中均未发现Treg细胞的表达。AAH组、HCC组中有Treg细胞的表达,且HCC组较AAH组增多(P<0.01)。在癌旁组织中已有Treg细胞浸润,但较肝癌组织中Treg细胞数量少(P<0.01)。肝癌组织中不同患者性别、年龄、术前AFP水平的Treg细胞数量差异无统计学意义,而在不同肿瘤大小、肿瘤包膜是否完整及术前HBV-DNA水平是否升高中Treg细胞数量差异有统计学意义(P<0.05或P<0.01)。结论:Treg细胞的表达与肿瘤的发生和发展有关,在肿瘤免疫中起负调节作用。

Objective： To investigate the distribution of CD^4＋ CD25^＋ regulatory T cells（Treg） in different liver tumors, and the role in the process of tumor occurrence and development. Methods： According to the pathological result, 80 patients were divided into focal nodular hyperplasia group （FNH, n= 10）, atypical adenomatous hyperplasia group （AAH, n= 10） and hepatocellular carcinoma group （HCC, n=60）. Another 10 cases of normal liver tissue （liver hemangioma edge of the liver） were selected for the control group. The expression of Treg cells in different tumor slices was detected by double ELISA of immunohistochemical staining. The expression of Treg cells was compared between FNH, AAH and HCC groups. The influencing factors of Treg cells were analyzed between groups in HCC groups. Results： There was no expression of Treg cells in normal liver tissue and FNH group. There were positive expressions of Treg cells in AAH and HCC group, and there was a significantly higher expression of Treg cells in HCC group than that of AAH group （P 〈 0.01）. There was Treg cell infiltration in tumor adja- cent tissues, but which was lower than that in tumor tissue （P 〈 0.01）. The expression Of Treg cells was no significant difference between gender, age and preoperative alpha-fetoprotein （AFP） value （P 〉 0.01）. There were significant differences in the number of Treg ceils between size of tumor, the tumor capsule and preoperative HBV-DNA level （P 〉 0.05 or P 〉 0.01）. Conclusion： The expression of Treg cells was correlated with the occun＇ence and development of the tumor. It plays a negative regulatory role in the immune of the tumor.