保加利亚乳杆菌冻干保护剂的研究

Study on cryoprotectants for Lactobacillus bulgaricus

采用正交试验研究了保加利亚乳杆菌在冷冻干燥条件下的最佳保护剂的筛选。结果表明:在所选的冷冻干燥保护剂中,VC影响极显著,脱脂乳和谷氨酸钠影响显著,明胶影响不显著;筛选出最佳复合保护剂组成为脱脂乳10%、VC0.5%、谷氨酸钠0.5%、明胶1.5%。

By an orthogonal experiment design, the selection of the optimum cryoprotectants was studied for Lactobacillus bulgaricus. The results showed that, among the cryoprotectants studied, VC was extremely remarkable in effect, skimmed milk and sodium glutamate were remarkable, and gelatin not remarkable. The optimal composite cryoprotectant was composed of 10% skimmed milk, 0.5% VC, 0.5% sodium glutamate （MSG） and 1.5% gelatin.