摘要
本文建立了水产品中10种大环内酯类抗生素的高效液相色谱-电喷雾串联质谱检测方法。对比几种检测方法后,最终采用乙腈进行提取的方法。在5.0~200.0μg/L范围内,10种大环内酯类抗生素的线性相关系数均大于0.99。通过实际样品的添加回收试验,方法的定量下限(S/N=10)为1.0μg/kg,检出限(S/N=3)为0.2μg/kg,3个添加水平中,龙虾、鮰鱼的加标回收率分别为69.4%~115.3%和63.1%~100.9%,日内相对标准偏差(RSD)分别为0.73%~3.39%及0.8%~4.44%。结果表明,该法简单、灵敏、特异性强,适用于水产品中大环内酯类药物残留的分析确证。
Determination of 10 kinds of Macrolide durgs by high performance liquid chromatography tandem mass spectrometry ( HPLC - MS/MS) in aquatic products has been studied in this research. Contrast several extraction methods, samples finally were extracted by acetonitrile. In the range of 5.0 - 200.0μg/L, the linear correlation coefficients for the determination for the above ten kinds of macrolide antibiotics were greater than 0.99. The detection quality (S/N = 10) and the detection limit (S/N = 3 ) were 1.0 μg/kg and 0.2 p,g/kg, respectively. Recoveries for the lobster, catfish of fish, honey and milk were 69.4% - 115.3 % ,63.1% to 100.9% , RSD in the day were 0.73% - 3.39% and 0. 8% - 4.44%. Results indicated that the established method is simple, sensitive, and specific for the determination of macrolide residues in aquatic products.
出处
《中国兽药杂志》
2012年第4期29-33,共5页
Chinese Journal of Veterinary Drug
基金
国家质检总局科研计划项目(No.2010KJ38)