扩展莫尼茨绦虫烯醇化酶基因的原核表达及抗原性鉴定

Cloning and prokaryotic expression and antigenicity identification of enolase from Moniezia expansa

为表达扩展莫尼茨绦虫(Moniezia expansa)的烯醇化酶(Enolase),本研究采用RT-PCR方法以M.expansa组织总RNA为模板扩增enolase基因,将其克隆到原核表达载体pET-28a中,并转化BL21(DE3)感受态细胞,以IPTG进行诱导表达。SDS-PAGE结果显示表达的重组Enolase约为47 ku。Western blot分析表明,天然蛋白能够被重组表达产物免疫小鼠的血清识别,出现两条免疫印记条带,即天然蛋白以两种形式(约47 ku和40 ku)存在,并具有抗原性,为该蛋白功能研究奠定了基础。

To clone and express the coding enolase gene from Moniezia expansa. One pair of specific primers was designed according to M. expansa enolase cDNA in Genbank （JF803807）. Total RNA was extracted from M. expansa and the enolase gene was amplified by RT-PCR and cloned into pET28a vector. The recombinant plasmid pET28a-Enolase was transformed into E. coli BL21 （DE3） and induced with IPTG for expression. The results showed that the recombinant enolase was about 47 ku and two protein bands （about 47 ku and 40 ku） were detected in the natural enolase with positive serum prepured from mice. Those results would be useful to further study of the enolase function immuned with the recombinant protein. This is the base of researching into enolase gene fimction and genetic engineering application.