中国沿海地区汉族男性人群白细胞介素-1β-511C/T基因多态性与痛风的易感性研究

Relationship between interleukin-1β-511C/T polymorphism and susceptibility to gout in Chinese malepopulation

目的探讨中国山东省沿海地区汉族男性群体的白细胞介素（IL）-1β--511位点的多态性是否与痛风的易感性有关。方法采用聚合酶链反应．限制性片段长度多态性（PCR-RFLP）检测276例痛风患者和268名健康对照者的IL-1β-511位点的基因多态性分布。统计学处理采用矿检验和t检验。结果IL-1β启动子区-511位点CC、CT和TT基因型频率在痛风组与健康对照组相比差异无统计学意义（r=3．251，P=0．197，df=2）；2组的等位基因频率C和T间差异也无统计学意义坼=2．941，P=0．086，0R=1．232，95％C1=0．971-1．563）。经X^2检验，IL-1β基因-511C/T点基因多态性与痛风病的危险因素无相关性。痛风组与健康对照组相比，具有较高的体质量指数（BMI）、血压水平、血尿酸、血糖、甘油三酯及胆固醇水平，差异有统计学意义（P〈0．01）。结论中国沿海地区汉族男性人群中IL-1β-511C／T基因多态性与痛风的易感性无关联性。

Objective To explore gene polymorphism of the C/T genotype of rs 16 944 in the promo- ter of IL-I~ gene in male population living in the coastal area of Shandong, and thus to investigate the relationship between the gene polymorphism of IL-1β and gout. Methods A total of 276 gout patients and 268 healthy controls were enrolled. The possible association between the polymorphism of IL-1β-511 C/T and gout in Chinese was investigated and genotype frequencies and allelic frequencies were calculated by polymerase chain reaction （PCR） restriction fragment length polymorphism （RFLP） method. Hardy Weinberg was used to verify the representativeness of the sample. Comparisons between the groups were performed with X2 test and t test. Results There was no statistically difference in IL-1β-511 C/T genotype frequencies between gout patients and controls（x^2=3.251, P=0.197, df=2）. The allele frequencies of C and T in gout cases were not different from those in the controls （A=2.941, P=0.086, OR=1.232, 95%CI： 0.971-1.563）. Moreover, no association between IL-1β-511C/T genotypes and risk factors for gout were observed in gout cases by test. Conclusion Results of the present study suggest that the C/T genotype of rs 16944 in the promoter of IL-1β gene is not associated with gout in male population living in the coastal area of Shandong.