川芎嗪体内抗血栓活性及机制探究

Effect and mechanism of tetramethylpyrazine on antithrombotic

目的:研究川芎嗪抗血栓作用及机制。方法:观察大鼠灌胃给予川芎嗪(20、40和80mg/kg)后对下腔静脉结扎和电刺激致血栓形成的影响,同时测定家兔灌胃给予的川芎嗪(10、20和40mg/kg)后对凝血酶、二磷酸腺苷(ADP)、胶原诱导的血小板聚集率、非闭塞性静脉血栓形成、凝血酶抑制率和对血浆凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)的影响。结果:川芎嗪对凝血酶、ADP诱导的血小板聚集均有抑制作用,但对胶原诱导的抑制能力较弱。川芎嗪有效地减少实验动物动静脉血栓的形成,在体内与凝血酶也有一定的亲和力,且能较好地延长PT。结论:川芎嗪具有一定的抗血栓作用,其机制可能与保护内皮与抑制血小板聚集有关。

To investigate the effect and mechanism of tetramethylpyrazine on antithrom- botic. METHODS： The effect of tetrameth- ylpyrazine （20, 40, 80 mg/kg, i. g. ） on throm-bosis induced by inferior vena cava ligation and electrically mediated carotid arterial in rats were evaluated. And studies were also conducted in rate of platelet aggregation, non-occlusion thro-mbosis, thrombin inhibition and plasm pro-time prothrombin time （PT） and activated partial thromboplastin time （APTT） in rabbits of dif- ferent does of tetramethylpyrazine （10, 20, 40 mg/kg, i.g. ）. RESULTS： Tetramethylpyrazine showed competent anti-thrombin activity and in- hibited platelet aggregation induced by thrombin and ADP, but presented lower potency in plate- let aggregation induced by collagen. In addition it markedly decreased venous and arterial throm-bosis and extended ethylpyrazine has PT. CONCLUSION ： a significant effect tithrombosis in vivo. The mechanism be related to the inhibition tion and protection of endot of platelet helium. Tetram- on an- seems to aggrega-