注射用羟基红花黄色素A对家兔血小板聚集、超微结构及血小板活化因子诱导后富血小板血浆中GMP-140含量的影响

Effects of HSYA injection on platelet aggregation,ultrastructure and the content of PAF induced GMP-140 in PRP in rabbits

目的:观察注射用羟基红花黄色素A(HSYA)对家兔血小板聚集功能及超微结构的影响。方法:采用体内实验法,观察3种剂量的注射用HSYA(10、5和2.5mg/kg)对由花生四烯酸(AA)、二磷酸腺苷(ADP)和血小板活化因子(PAF)(3.6nmol/L)诱导的家兔血小板聚集作用的影响,以及血小板超微结构的变化;并单独研究了以PAF为诱导剂的情况下,富血小板血浆(PRP)中α-颗粒膜蛋白-140(GMP-140)的含量。结果:各剂量HSYA注射剂能抑制AA、PAF诱导的家兔血小板聚集(P<0.05,P<0.01);扫描电镜显示:各剂量HSYA注射剂能减少AA和PAF诱导后的聚集型血小板数量并使树突型血小板突起变少变短(P<0.05,P<0.01);另外,各剂量HSYA注射剂还能降低经PAF诱导后GMP-140的含量(P<0.01)。结论:注射用羟基红花黄色素A具有显著的抗PAF诱导的血小板聚集作用,抑制血小板的活化,从而为临床抗血小板聚集药物的使用提供更多选择。

To observe the effects of HSYA injection on platelet aggregation and ultra structure in rabbits. METHODS： In vivo experi- ments, when platelet activating factor （PAF） induced respectively, we observed the impacts of HSYA injection （10 mg/kg, 5 mg/kg and 2. 5 mg/kg） on platelet aggregation, ultra structural changes of platelets and PAF-induced content of GMP-140. RESULTS： HSYA injection inhibited AA and PAF-induced platelet aggregation（P〈 0.05,P〈 0.01）. Scanning electron microscope showed： HSYA injection could reduce the num- ber of aggregation type plateles which were in- duced by AA and PAF; and make fewer andshorter processes of dendritic platelets （P 0. 05 , P〈0. 01）. HSYA injection can reduce the content of GMP-140 in PRP（P〈0.01）. CON- CLUSION： HSYA injection can antagonism PAF-induced platelet aggregation, inhibit the activation of platelets and reduce the content of GMP-140. Thus for, there will be more choices for clinical using of anti-platelet aggregation drugs.