摘要
目的研究乳腺癌MCF-7细胞的分子生物学特征。方法采用甲基化特异性PCR(MSP),对MCF-7细胞进行Stratifin和CyclinD2基因甲基化检测;采用RT-PCR法,检测Stratifin、CyclinD2和Dnmt3b基因的mRNA表达。结果乳腺癌MCF-7细胞系中,Stratifin基因是非甲基化的,mRNA表达水平较高;CyclinD2基因完全甲基化,mRNA表达缺失;Dnmt3b基因呈明显表达。结论 Stratifin和CyclinD2基因的甲基化发生,可能下调其mRNA的转录表达,MCF-7细胞的这些分子生物学特性可为乳腺癌的表观遗传学研究提供一定的参考价值。
Objective To study the molecular characteristics of breast cancer cell line MCF-7. Methods Methylations of Stratifin and CyclinD2 genes were examined by methylation-specific polymerase chain reaction（MSP）. Expressions of Stratifin, CyclinD2 and Dnmt3b genes mRNA were detected by RT-PCR. Results Unmethylation and high mRNA level of Stratifin gene, complete methylation and expression deletion of CyclinD2 gene, significant expression of Dnmt3b gene were showed in breast cancer cell line MCF-7. Conclusion Methylations of Stratifin and CyclinD2 genes maybe down-regulate mRNA expressions. These molecular characteristics provide certain reference to epigenetic study of breast cancer.
出处
《实用癌症杂志》
2012年第2期118-119,共2页
The Practical Journal of Cancer
基金
广东省深圳市科技计划项目(201103029)
