芳香化酶抑制剂对卵巢癌组织块体外培养活性的影响

Effects of Aromatase Inhibitors on the Activity of Ovarian Cancer Histocultur

目的建立卵巢癌组织块体外培养模型,探讨芳香化酶抑制剂(aromatase inhibitors,AIs)来曲唑对卵巢癌组织块体外培养活性的影响。方法选取卵巢浆液性囊腺癌组织块进行体外培养,加入不同浓度的来曲唑观察肿瘤细胞坏死情况。采用免疫组织化学方法,检测标本中芳香化酶P450及雌激素受体的表达。结果建立卵巢癌组织块体外培养模型,并在6例标本中筛选出AIs敏感病例及部分敏感病例各1例。敏感及部分敏感病例芳香化酶P450及雌激素受体的表达水平均与不敏感病例无明显差别。结论卵巢癌组织块体外培养,为卵巢癌的体外药敏试验提供了一个良好的研究模型,来曲唑对部分卵巢癌肿瘤组织有抑制作用。

Objective To establish ovarian cancer histoculture model and explore the effects of aromatase inhibitor letrozole on the activity of ovarian cancer histoculture.Methods Histoculture of ovarian serous cystadenoma join in different levels of letrozole to observe the tumor cells necrosis rate changes.Immunohistochemistry staining was used to check the presence and the features of aromatase and estrogen receptor of specimens.Results Establish ovarian cancer histoculture model,filter out a sensitive case and a partly sensitive case to aromatase inhibitors in 6 cases.The expression of aromatase and estrogen receptor in the two cases were no obvious difference.Conclusion ovarian cancer histoculture is a good mode of ovarian cancer chemosensitivity assay.Letrozole is a inhibition for some cases of ovarian cancer.