红花中羟基红花黄色素A的分离纯化工艺

Separation and Purification Technology of Hydroxysafflor Yellow A from Carthamus tinctorius

目的:分离纯化红花中羟基红花黄色素A(HSYA)。方法:采用ZTCⅡ澄清剂进行初步除杂,以吸附量、吸附率和解吸附率为指标,筛选分离HSYA的大孔树脂型号、工艺以及聚酰胺树脂纯化HSYA的工艺。结果:选取X-5型大孔树脂,分离工艺要求吸附流速为1mL·min-1,最佳吸附pH值为3,上柱量为柱体积的2%,洗脱溶剂为50%乙醇溶液。聚酰胺树脂纯化工艺要求径高比1∶14,上柱量为柱体积的2%,流速为1.5mL·min-1,洗脱溶剂为乙酸乙酯-甲醇-3.6%盐酸(1∶3∶6),反复上柱6次。得到HSYA的产率为25.63%,纯度为83.65%。结论:所选工艺成本低、所获产品纯度较高,可作为获得HSYA的有效方法。

OBJECTIVE：To separate and purify hydroxysaffl yellow A（HSYA）from Carthamus tinctorius.METHODS：HSYA was separated from C.tinctorius and purified by using ZTCⅡ as clarifier.The type model of macroporous resin,separation technology and purification technology were screened with adsorption quantity,adsorption rate and desorption rate as index.RESULTS：X-5 macroporous resin was adopted.The separation technology was as follows：absorptive flow rate of 1 mL·min-1,the best absorptive pH value of 3,2% of column volume,50% ethanol as elution solvent.The purification technology of polyamide resin was as follows：diameter-height ratio of 1 ∶ 14,2% of column volume,flow rate of 1.5 mL·min-1,ethyl acetate-methanol-3.6% hydrochloric acid（1 ∶ 3 ∶ 6）as elution solvent,6 times.The yield of HSYA was 25.63% and the purity of it was 83.65%.CONCLUSION：The technology is cheap and high purity in product.It is effective method to prepare HSYA.