10日龄肉鸭混合感染新型鸭肝炎病毒和多杀性巴氏杆菌的病原学研究

Isolation and identification of new serotype duck hepatitis virus and Pasteurella multocida from 10-day-old meat ducklings

对1例疑似鸭肝炎病毒和多杀性巴氏杆菌混合感染的10日龄肉鸭采用常规的病毒、细菌鉴定方法和RT-PCR、PCR方法分别进行病毒、细菌的分离与鉴定。病毒鉴定为新型鸭肝炎病毒,细菌鉴定为荚膜血清A型多杀性巴氏杆菌多杀亚种。细菌对SPF鸡的毒力试验结果显示,分离的巴氏杆菌与强毒标准株C48-1毒力相近,为强毒株。细菌对10日龄肉鸭的致病性回归试验结果表明,一定数量的该株巴氏杆菌可导致10日龄雏鸭的感染死亡。结果表明,该批肉鸭为新型鸭肝炎病毒和A型多杀性巴氏杆菌混合感染。这是国内首例从感染鸭肝炎病毒10日龄雏鸭肝脏中分离到多杀性巴氏杆菌。

This experiment was conducted to identify the pathogen of 10-day-old meat ducklings which was suspected of infecting duck hepatitis virus and Pasteurella multocida（P.mutocida） simultaneously.The conventional test of virus and bacterium,RT-PCR and PCR methods were all used to identify the viral isolate and bacterial isolate respectively.Results indicated that the isolated virus is new serotype duck hepatitis virus,the isolated bacterium is P.multocida subsp.mutocida serotype A.In animal pathogenicity test,P.multocida isolate is similar in virulent to national standard velogen strain C48-1,the result of pathogenicity test on 10-day-old meat ducklings demonstrated that Pasteurella multocida is lethiferous to 10-day-old ducklings in certain CFUs.The meat ducklings were infected by new serotype duck hepatitis virus and Pasteurella multocida subsp.mutocida serotype A simultaneously.This is the first report that Pasteurella multocida was isolated from 10-day-old ducklings which affected new serotype duck hepatitis virus in China.