日粮铬对热应激种公鸡肾脏组织结构及血清抗氧化功能的影响

Effect of adding chrome in diets on kidney tissue structure and oxidation prevention function in serum of breeder cocks under heat stress

选用72只35周龄罗曼褐种公鸡,随机分为6组,每组12只,对照组饲喂基础日粮,分别在基础日粮中添加0.2、0.4、0.6、0.8、1.0mg/kg铬量的酵母铬,作为试验Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ组,在热应激条件下(日:最高温度37.5℃,最低温度28.0℃,平均温度31.7℃)饲养45d。结果显示,在热应激条件下,给种公鸡日粮中添加一定量的酵母铬不仅能有效降低热应激对种公鸡肾脏组织的损伤,而且可显著提高血清中GSH-Px、SOD和CAT活性,降低血清中MDA含量(P<0.05或P<0.01)。结果表明,夏季高温环境下,在种公鸡日粮中添加0.8～1.0mg/kg铬量的酵母铬可通过增强机体的抗氧化功能,抑制机体内自由基和脂质过氧化物的产生,保护机体组织细胞膜的完整,维持机体正常生理功能。

The design was used to study the effect of adding chrome in diets on kidney tissue structure and oxidation prevention in serum of breeder cocks under heat stress.72 cocks,35-week-old,were divided randomly into 6 groups.The control group was fed with the basal diet,and the treatment groups were given the diet supplemented with 0.2,0.4,0.6,0.8,1.0 mg chrome,respectively,these cocks were raised under heat stress（day：the uppermost temperature 37.5℃,the lowermost temperature 28.0℃,the average temperature 31.7℃） for 45 day.The results showed： the diet adding some chrome not only can efficiently respite damage of heat stress in kidney tissue structure of breeder cocks,but also rises GSH-Px,SOD and CAT in serum,decreases MDA in serum of breeder cocks under heat stress（P〈0.05 or P〈0.01）.The results hint that in high temperature condition of summer,the diet adding 0.8-1.0 mg/kg chrome strengthens body＇s oxidation prevention function,restraints ROS in body,protects cell membrane integrity.