摘要
目的:观察氧化低密度脂蛋白(oxygenized low density lipoprotein,oxLDL)和脂蛋白相关磷脂酶A2(lip-oprotein associated phospholipase A2,Lp-PLA2)短发夹RNA(shRNA)对小鼠单核巨噬细胞(RAW264.7)Lp-PLA2活性、mRNA表达及炎症基因表达的影响。方法:采用0、20、40、60和80μg/ml oxLDL刺激RAW264.7细胞并用不同剂量Lp-PLA2shRNA转染oxLDL 60μg/ml处理组,应用ELISA、逆转录-聚合酶链反应检测Lp-PLA2、单核细胞趋化蛋白-1(Monocyte Chemoattractant Protein-1,MCP-1)及其mRNA表达的影响。结果:RAW264.7细胞经oxLDL刺激后Lp-PLA2表达明显升高,且呈时间和剂量依赖性。Lp-PLA2shRNA转染小鼠单核巨噬细胞后,炎症基因及mRNA表达明显下降。结论:Lp-PLA2shRNA转染可明显逆转oxLDL刺激引起的RAW264.7细胞炎症基因高表达。
Objective： To identify the effects of lipoprotein associated phospholipase A2（Lp-PLA2） shRNA and oxygenized low density lipoprotein（oxLDL） on the expression of Lp-PLA2 and inflammatory gene expression in mouse monocyte-macrophages. Methods： After 0,20,40,60 and 80μg/ml oxLDL and 60μg/ml oxLDL with different dosage of Lp-PLA2 shRNA treating RAW264.7 cells for 12 hours,Lp-PLA2 and inflammatory gene expression were detected by ELISA kit,and mRNA level of Lp-PLA2 and MCP-1 were detected by RT-PCR. Results： oxLDL increased the activity of Lp-PLA2 and its mRNA expression in a dose and concentration dependent manner,Lp-PLA2 shRNA at varied concentration could inhibit Lp-PLA2 and MCP-1 activity and its mRNA expression induced by oxLDL. Conclusion： Lp-PLA2 shRNA could inhibit Lp-PLA2 and inflammatory gene expression evoked by oxLDL.
出处
《河南医学研究》
CAS
2012年第1期4-7,共4页
Henan Medical Research
