PTEN和LIVIN在肾透明细胞癌中的表达及临床意义

Expression and clinical significance of PTEN and LIVIN in renal clear cell carcinomas

目的:研究双特异磷酸酶活性的磷酸脂酶基因(PTEN)和黑色素瘤凋亡抑制蛋白或肾凋亡抑制蛋白(LIVIN)在肾透明细胞癌中的表达及其相关性。探讨与肾透明细胞癌临床分期及病理分级关系。方法:应用免疫组化SP法检测50例肾脏透明细胞癌、25例癌旁组织(距肿瘤边缘>1 cm)及10例正常肾组织中PTEN及LIVIN的表达。结果:PTEN在肾透明细胞癌中的阳性率明显低于癌旁及正常肾组织,差异有统计学意义(P<0.01);LIVIN在正常肾组织及癌旁组织无表达,而在肾透明细胞癌中高表达,差异有统计学意义(P<0.01)。在不同临床分期的肾透明细胞癌组织中PTEN和LIVIN的表达有显著差异性(P<0.01);并且两因子的表达呈显著负相关(r=-0.547,P<0.01)。结论:PTEN和LIVIN呈负相关性,在肾透明细胞癌的发生发展中起重要的作用,两个因子联合检测可作为判定肾透明细胞癌恶性程度的重要指标。

Objective： To study the expression of PTEN,LIVIN in human renal clear cell carcinomas（Rccc）and to investigate their relationship. Methods： We detected the expression of PTEN and LIVIN in 50 cases of Rccc tissue,25 cases of para carcinoma and 10 cases of normal renal tissues by using immunohistoch emistrical SP method,and analyzed the relationship between the expression and the biological behavior,such as histological grade,lymph node metastases and clinical staging. Results： The positive rate of PTEN in Rccc significantly lower than that in renal tissue adjacent to carcinoma tissue and normal renal tissue,with statistical significance（P〈0.01）.LIVIN in renal tissue adjacent to carcinoma tissue and the normal renal tissue displays deletion,whole in Rccc,it is high expression,with statistical significance（P〈0.01）.With the increasing of the clinical stage in Rccc,the positive rate of PTEN gradually decreased but the LIVIN gradually increased,with statistical significance（P〈0.01）.The expression of PTEN and LIVIN were negatively correlated（r=-0.395,P〈0.01）. Conclusion： PTEN and LIVIN in renal clear cell carcinoma play an important role in the development.